PMID- 8613705
OWN - NLM
STAT- MEDLINE
DCOM- 19960604
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 59
IP  - 4
DP  - 1996 Apr
TI  - Estrogen regulation of JE/MCP-1 mRNA expression in fibroblasts.
PG  - 562-8
AB  - We have recently demonstrated that 17 beta-estradiol (E2) inhibits peritoneal 
      adhesion formation. Because macrophages play a central role in inflammation and 
      wound healing, we chose to investigate whether the E2 could inhibit the 
      expression of JE, the murine monocyte chemoattractant protein-1 (MCP-1). To 
      accomplish this, murine fibroblasts were cultured with physiological 
      concentrations of E2 (3-300 pg/ml) with or without inducers of JE/MCP-1 mRNA 
      expression. Untreated cells failed to express the message, but, following 
      stimulation, a marked increase in JE/MCP-1 mRNA expression was observed. At 10-30 
      pg/ml, E2 had no effect on JE/MCP-1 mRNA expression in stimulated fibroblasts. In 
      contrast, lower and higher doses of E2 inhibited the expression of JE/MCP-1 mRNA 
      in stimulated fibroblasts. Treatment with tamoxifen reversed the E2-inhibition of 
      expression of the message. These data demonstrate that JE/MCP-1 mRNA expression 
      is controlled, in part, by estrogen and suggest that macrophage recruitment may 
      be affected by circulating levels of E2.
FAU - Kovacs, E J
AU  - Kovacs EJ
AD  - Department of Cell Biology, Loyola University, Maywood, IL, USA.
FAU - Faunce, D E
AU  - Faunce DE
FAU - Ramer-Quinn, D S
AU  - Ramer-Quinn DS
FAU - Mott, F J
AU  - Mott FJ
FAU - Dy, P W
AU  - Dy PW
FAU - Frazier-Jessen, M R
AU  - Frazier-Jessen MR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Chemokine CCL2)
RN  - 0 (Estrogen Antagonists)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (RNA, Messenger)
RN  - 094ZI81Y45 (Tamoxifen)
RN  - 4TI98Z838E (Estradiol)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - 3T3 Cells/drug effects/metabolism/physiology
MH  - Animals
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis/genetics
MH  - Dexamethasone/pharmacology
MH  - Estradiol/*pharmacology
MH  - Estrogen Antagonists/pharmacology
MH  - Fibroblasts/*drug effects/*metabolism/physiology
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Mice
MH  - Platelet-Derived Growth Factor/antagonists & inhibitors/pharmacology
MH  - RNA, Messenger/genetics/*metabolism
MH  - Sensitivity and Specificity
MH  - Tamoxifen/pharmacology
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
AID - 10.1002/jlb.59.4.562 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1996 Apr;59(4):562-8. doi: 10.1002/jlb.59.4.562.