PMID- 8613800
OWN - NLM
STAT- MEDLINE
DCOM- 19960603
LR  - 20191101
IS  - 0270-6474 (Print)
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 16
IP  - 1
DP  - 1996 Jan
TI  - Medial preoptic area afferents to periaqueductal gray medullo-output neurons: a 
      combined Fos and tract tracing study.
PG  - 333-44
AB  - We have shown recently that the medial preoptic area (MPO) robustly innervates 
      discrete columns along the rostrocaudal axis of the midbrain periaqueductal gray 
      (PAG). However, the location of PAG neurons responsive to MPO activation is not 
      known. Anterograde tract tracing was used in combination with Fos 
      immunohistochemistry to characterize the MPO --> PAG pathway anatomically and 
      functionally within the same animal. Focal electrical or chemical stimulation of 
      MPO in anesthetized rats induced extensive Fos expression within the PAG compared 
      with sham controls. Fos-positive neurons were organized as 2-3 longitudinal 
      columns. The organization and location of these columns overlapped remarkably 
      well with the distribution of fibers and terminals in PAG labeled by Phaseolus 
      vulgaris leucoagglutinin (PHA-L) injected into the same MPO stimulation site. 
      This indicates that MPO inputs may terminate on the soma or proximal dendrites of 
      neurons exhibiting elevated Fos. A second series of experiments investigated 
      whether MPO stimulation excited PAG neurons with descending projections to the 
      medulla. Retrograde labelling of PAG neurons projecting to the medial and lateral 
      regions of the rostroventral medulla (RVM) was combined with MPO-induced Fos 
      expression. The results showed that a substantial population (37-53%) of 
      Fos-positive PAG neurons projected to the ventral medulla. This indicates that 
      MPO stimulation engages PAG-medullary output neurons. Taken together, these 
      results suggest that the MPO --> Pag --> RVM projection constitutes a functional 
      pathway. This circuit may coordinately regulate neuroendocrine, motor, and 
      autonomic adjustments necessary for the elaboration of sexual behaviors.
FAU - Rizvi, T A
AU  - Rizvi TA
AD  - Department of Anatomy, University of Maryland School of Medicine, Baltimore 
      21201, USA.
FAU - Murphy, A Z
AU  - Murphy AZ
FAU - Ennis, M
AU  - Ennis M
FAU - Behbehani, M M
AU  - Behbehani MM
FAU - Shipley, M T
AU  - Shipley MT
LA  - eng
GR  - NS20643/NS/NINDS NIH HHS/United States
GR  - NS24698/NS/NINDS NIH HHS/United States
GR  - NS29218/NS/NINDS NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Phytohemagglutinins)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (Wheat Germ Agglutinins)
RN  - 0 (leukoagglutinins, plants)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Afferent Pathways
MH  - Animals
MH  - Electric Stimulation
MH  - Gold
MH  - Male
MH  - Medulla Oblongata/*cytology
MH  - Neurons/*chemistry
MH  - Periaqueductal Gray/*cytology
MH  - Phytohemagglutinins
MH  - Preoptic Area/*cytology
MH  - Proto-Oncogene Proteins c-fos/*analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Wheat Germ Agglutinins
PMC - PMC6578705
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
PMCR- 1996/07/01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
PHST- 1996/07/01 00:00 [pmc-release]
AID - 10.1523/JNEUROSCI.16-01-00333.1996 [doi]
PST - ppublish
SO  - J Neurosci. 1996 Jan;16(1):333-44. doi: 10.1523/JNEUROSCI.16-01-00333.1996.