PMID- 8616028
OWN - NLM
STAT- MEDLINE
DCOM- 19960610
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 92
IP  - 3
DP  - 1996 Mar
TI  - Adult T-cell leukaemia/lymphoma with multiple integrations of human T-cell 
      lymphotropic virus type I proviral DNA: differing clinical features are linked to 
      varied proviral integration.
PG  - 632-8
AB  - Multiple integrations of human T-cell lymphotropic virus type I (HTLV-I) proviral 
      DNA are occasionally found in tumor cells from patients with adult T-cell 
      leukaemia/lymphoma (ATL). However, the clinical implications of multiple 
      integrations of HTLV-I in ATL have not been well established. We studied 95 
      patients with ATL to elucidate the relationship between the multiple integrations 
      of HTLV-I and the clinical characteristics. The proviral DNA of HTLV-I was 
      examined by standard Southern blot analysis using the probe of an entire HTLV-I 
      genome and the endonucleases with or without cleavage sites within the provirus. 
      Multiple integrations of HTLV-I were detected in eight patients as extraordinary 
      multiple bands; five patients showed multiple bands of the same intensity, and 
      the remaining three showed multiple bands of differing intensities. The patients 
      were divided into two groups based on these band patterns. One group was 
      considered to exhibit one tumour cell clone carrying multiple copies of the 
      provirus, whereas the other was considered to exhibit multiple tumour cell 
      clones, each carrying one copy of the provirus. The former group of patients 
      manifested a highly aggressive clinical course with frequent peculiar organ 
      infiltrations, including the retina, uvea and muscle, along with the presence of 
      large peripheral leukaemic T cells having flower-like nuclei. The latter group 
      demonstrated an indolent clinical course with skin lesions or small leukaemic T 
      cells having cleaved or lobulated nuclei. These findings suggest that the pattern 
      of multiple HTLV-integrations into the tumor cell(s) has clinical implications in 
      ATL. This may help to explain the heterogeneity of this disease.
FAU - Shimamoto, Y
AU  - Shimamoto Y
AD  - Division of Haematology, Department of Internal Medicine, Saga Medical School, 
      Japan.
FAU - Miyahara, M
AU  - Miyahara M
FAU - Yamada, H
AU  - Yamada H
FAU - Shibata, K
AU  - Shibata K
FAU - Matsuzaki, M
AU  - Matsuzaki M
FAU - Ono, K
AU  - Ono K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Blotting, Southern
MH  - DNA, Viral/*analysis
MH  - Female
MH  - Human T-lymphotropic virus 1/*genetics
MH  - Humans
MH  - Leukemia-Lymphoma, Adult T-Cell/*virology
MH  - Male
MH  - Middle Aged
MH  - Proviruses/genetics
MH  - Virus Integration
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
AID - 10.1046/j.1365-2141.1996.00376.x [doi]
PST - ppublish
SO  - Br J Haematol. 1996 Mar;92(3):632-8. doi: 10.1046/j.1365-2141.1996.00376.x.