PMID- 8616213
OWN - NLM
STAT- MEDLINE
DCOM- 19960613
LR  - 20190821
IS  - 0167-4412 (Print)
IS  - 0167-4412 (Linking)
VI  - 29
IP  - 6
DP  - 1995 Dec
TI  - Atypical phytochrome gene structure in the green alga Mesotaenium caldariorum.
PG  - 1127-42
AB  - The phytochrome photoreceptor in the green alga Mesotaenium caldariorum is 
      encoded by a small family of highly related genes. DNA sequence analysis of two 
      of the algal phytochrome genes indicates an atypical gene structure with numerous 
      long introns. The two genes, termed mesphy1a and mesphy1b, encode polypeptides 
      which differ by one amino acid in the region of overlap that was sequenced. 
      RT-PCR studies have established the intron-exon junctions of both genes and show 
      that both are expressed. RNA blot analysis indicates a single transcript of ca. 
      4.1 kb in length. The deduced amino acid sequence of the mesphy1b gene reveals 
      that the photoreceptor consists of 1142 amino acids, with an overall structure 
      similar to other phytochromes. Phylogenetic analyses indicate that the algal 
      phytochrome falls into a distinct subfamily with other lower plant phytochromes. 
      Profile analysis of an internal repeat found within the central hinge region of 
      the phytochrome polypeptide indicates an evolutionary relatedness to the 
      photoactive yellow protein from the purple bacterium Ectothiorhodospira 
      halophila, to several bacterial sensor kinase family members, and to a family of 
      eukaryotic regulatory proteins which includes the period clock (per) and 
      single-minded (sim) gene products of Drosophila. Since mutations which alter 
      phytochrome activity cluster within the region delimited by these direct repeats 
      (P.H. Quail et al., Science 268 (1995): 675-680), this conserved motif may play 
      an important role in the signal transducing function of these disparate protein 
      families.
FAU - Lagarias, D M
AU  - Lagarias DM
AD  - Section of Molecular and Cellular Biology, University of California, Davis 95616, 
      USA.
FAU - Wu, S H
AU  - Wu SH
FAU - Lagarias, J C
AU  - Lagarias JC
LA  - eng
SI  - GENBANK/D13519
SI  - GENBANK/L19596
SI  - GENBANK/L29642
SI  - GENBANK/M31067
SI  - GENBANK/U10325
SI  - GENBANK/U31283
SI  - GENBANK/U31284
SI  - GENBANK/X67684
SI  - GENBANK/Z22637
SI  - SWISSPROT/P05709
SI  - SWISSPROT/P08400
SI  - SWISSPROT/P10931
SI  - SWISSPROT/P10955
SI  - SWISSPROT/P13260
SI  - SWISSPROT/P16113
SI  - SWISSPROT/P25764
SI  - SWISSPROT/P26167
SI  - SWISSPROT/P26762
SI  - SWISSPROT/P29130
SI  - SWISSPROT/P30663
SI  - SWISSPROT/P33530
SI  - SWISSPROT/P37739
SI  - SWISSPROT/P39764
SI  - SWISSPROT/Q03353
SI  - SWISSPROT/Q04855
SI  - SWISSPROT/Q06067
GR  - 1 P41 RR06009/RR/NCRR NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Plant Mol Biol
JT  - Plant molecular biology
JID - 9106343
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (DNA Primers)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (PER protein, Drosophila)
RN  - 0 (Period Circadian Proteins)
RN  - 0 (sim protein, Drosophila)
RN  - 11121-56-5 (Phytochrome)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Bacteria/genetics
MH  - Base Sequence
MH  - Basic Helix-Loop-Helix Transcription Factors
MH  - Biological Evolution
MH  - Chlorophyta/*genetics
MH  - Consensus Sequence
MH  - DNA Primers
MH  - DNA-Binding Proteins/genetics
MH  - Drosophila/genetics
MH  - Drosophila Proteins
MH  - Exons
MH  - *Genes, Plant
MH  - Introns
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/genetics
MH  - Period Circadian Proteins
MH  - Phylogeny
MH  - Phytochrome/*genetics
MH  - Polymerase Chain Reaction/methods
MH  - Repetitive Sequences, Nucleic Acid
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
EDAT- 1995/12/01 00:00
MHDA- 1995/12/01 00:01
CRDT- 1995/12/01 00:00
PHST- 1995/12/01 00:00 [pubmed]
PHST- 1995/12/01 00:01 [medline]
PHST- 1995/12/01 00:00 [entrez]
AID - 10.1007/BF00020457 [doi]
PST - ppublish
SO  - Plant Mol Biol. 1995 Dec;29(6):1127-42. doi: 10.1007/BF00020457.