PMID- 8616572
OWN - NLM
STAT- MEDLINE
DCOM- 19960610
LR  - 20220317
IS  - 1073-449X (Print)
IS  - 1073-449X (Linking)
VI  - 153
IP  - 4 Pt 1
DP  - 1996 Apr
TI  - Increased MCP-1, RANTES, and MIP-1alpha in bronchoalveolar lavage fluid of 
      allergic asthmatic patients.
PG  - 1398-404
AB  - Chemokines are cytokines that induce chemotaxis of inflammatory cells. We studied 
      the presence of chemokines in bronchoalveolar lavage fluid (BALF) obtained from 
      nine allergic asthmatic patients and six nonsmoking normal individuals. The cells 
      were pelleted, and ribonucleic acid (RNA) was extracted by using RNAzol B. BALF 
      was assayed for monocyte chemoattractant protein-1 (MCP-1), regulated upon 
      activation in normal T cells, expressed, probably secreted (RANTES), macrophage 
      inflammatory protein-1alpha (MIP-1alpha) and interleukin-8 (IL-8) by 
      enzyme-linked immunosorbent assay (ELISA). The levels of MCP-1, RANTES, and 
      MIP-1alpha were significantly higher in the asthma patients than in the control 
      subjects (p<0.04). The concentrations of RANTES and MCP-1 correlated with the 
      lymphocyte count in the BAL specimens (r = 0.61 and 0.68, respectively). BALF 
      showed eosinophil chemotactic activity in vitro that was blocked by anti-RANTES 
      and anti-MCP-3 antibodies. The total cellular RNA was reverse-transcribed and the 
      complementary deoxyribonucleic acid (cDNA) was amplified with the polymerase 
      chain reaction (PCR) for MCP-1, MCP-3, RANTES, MIP-1alpha, IL-8, and beta-actin. 
      We found that messenger ribonucleic acids (mRNAs) for MCP-1, MCP-3, RANTES, 
      MIP-1alpha, and IL-8 were produced by BAL cells from most asthmatic and normal 
      subjects. We conclude that chemokines are produced in the airways, and that an 
      increased recovery of MCP-1, RANTES, and MIP-1alpha is observed in allergic 
      asthmatic patients.
FAU - Alam, R
AU  - Alam R
AD  - Department of Internal Medicine, University of Texas Medical Branch, Galveston, 
      USA.
FAU - York, J
AU  - York J
FAU - Boyars, M
AU  - Boyars M
FAU - Stafford, S
AU  - Stafford S
FAU - Grant, J A
AU  - Grant JA
FAU - Lee, J
AU  - Lee J
FAU - Forsythe, P
AU  - Forsythe P
FAU - Sim, T
AU  - Sim T
FAU - Ida, N
AU  - Ida N
LA  - eng
GR  - AI 27864/AI/NIAID NIH HHS/United States
GR  - MO1 RR 00073/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Respir Crit Care Med
JT  - American journal of respiratory and critical care medicine
JID - 9421642
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Interleukin-8)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (Monokines)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Asthma/*metabolism/physiopathology
MH  - Base Sequence
MH  - Bronchoalveolar Lavage Fluid/*chemistry
MH  - Chemokine CCL2/*analysis/physiology
MH  - Chemokine CCL3
MH  - Chemokine CCL4
MH  - Chemokine CCL5/*analysis/physiology
MH  - Female
MH  - Humans
MH  - Interleukin-8/analysis
MH  - Lymphocyte Count
MH  - Macrophage Inflammatory Proteins
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Monokines/*analysis/physiology
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
AID - 10.1164/ajrccm.153.4.8616572 [doi]
PST - ppublish
SO  - Am J Respir Crit Care Med. 1996 Apr;153(4 Pt 1):1398-404. doi: 
      10.1164/ajrccm.153.4.8616572.