PMID- 8618444
OWN - NLM
STAT- MEDLINE
DCOM- 19960613
LR  - 20130304
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 10
IP  - 4
DP  - 1996 Apr
TI  - Clonal cell lineage involvement in myelodysplastic syndromes studied by 
      fluorescence in situ hybridization and morphology.
PG  - 662-8
AB  - We have used DNA fluorescence in situ hybridization (FISH) in combination with 
      morphology to study cell lineage involvement in peripheral blood and bone marrow 
      smears from 15 patients with myelodysplastic syndromes (MDS) and known numerical 
      chromosomal aberrations. Eleven cases were investigated at diagnosis of MDS, and 
      four at transformation to acute myeloid leukemia (MDS-AML). Using conventional 
      cytogenetics, monosomy 7 was detected in nine cases, monosomy 17 in two, and 
      trisomy 8 in five, either as a single aberration or as part of a complex clone. 
      One case had both monosomy 7 and 17. Three biotinylated DNA probes directed 
      against the pericentromeric region of chromosomes 7, 8 and 17, respectively, were 
      used. Bone marrow smears were first stained with May-Grunewald-Giemsa (MGG) for 
      morphologic evaluation, and regions of interest documented by photography. Later 
      the same regions were targeted after hybridization, which allowed FISH analysis 
      of selected bone marrow cells. In each patient between 267 and 921 cells (mean 
      453) were studied. In most cases a majority of the non-lymphoid bone marrow cells 
      appeared to be of clonal origin, ie showed either monosomy or trisomy by FISH, 
      while in contrast both lymphocytes and plasma cells generally were disomic, ie 
      displayed two fluorescent spots for each probe. The percentages of clonal bone 
      marrow cells differed greatly between patients, but in a single case there was a 
      good agreement between the extent of granulocytic, monocytic and erythrocytic 
      cell lineage involvement. In relation to the clinical stage of MDS, patients with 
      more advanced disease had a significantly higher mean percentage of bone marrow 
      blasts showing monosomy or trisomy, while disomic, possibly non-clonal cells were 
      more frequent among the earlier forms of MDS. In the four cases of MDS-AML nearly 
      all non-lymphoid bone marrow cells belonged to the abnormal clone. The FISH 
      technique offers information regarding the distribution of clonal and non-clonal 
      bone marrow cells in MDS, which might have prognostic and possibly, therapeutic 
      implications.
FAU - Bernell, P
AU  - Bernell P
AD  - Department of Medicine, Karolinska Institue, Danderyd Hospital, Sweden.
FAU - Jacobsson, B
AU  - Jacobsson B
FAU - Nordgren, A
AU  - Nordgren A
FAU - Hast, R
AU  - Hast R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (DNA Probes)
SB  - IM
MH  - Acute Disease
MH  - Aged
MH  - Aged, 80 and over
MH  - Bone Marrow/pathology
MH  - Cell Differentiation
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 7
MH  - *Chromosomes, Human, Pair 8
MH  - DNA Probes
MH  - Female
MH  - Granulocytes/pathology
MH  - Hematopoietic Stem Cells/*pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Myeloid/blood/genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - *Monosomy
MH  - Myelodysplastic Syndromes/blood/*genetics/pathology
MH  - Reference Values
MH  - *Trisomy
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
PST - ppublish
SO  - Leukemia. 1996 Apr;10(4):662-8.