PMID- 8619129
OWN - NLM
STAT- MEDLINE
DCOM- 19960613
LR  - 20191024
IS  - 0740-7750 (Print)
IS  - 0740-7750 (Linking)
VI  - 21
IP  - 5
DP  - 1995 Sep
TI  - The XIST locus replicates late on the active X, and earlier on the inactive X 
      based on FISH DNA replication analysis of somatic cell hybrids.
PG  - 327-33
AB  - We have recently reported results of DNA replication analysis of three X-linked 
      loci (FRAXA, F8C and XIST) on the X chromosomes in male and female fibroblasts 
      using fluorescence in situ hybridization (FISH) (1). Although our findings that 
      XIST replicates later on the active X than on the inactive X are similar to those 
      of Boggs & Chinault (2) based on a FISH assay in female lymphoblasts, they are 
      the opposite of observations recently reported by Hansen et al. (3) using a 
      different technique. Because our conclusions about the inactive X were deduced 
      from the behavior of the active X in male cells, we reexamined the time when 
      these loci replicate on the human inactive X chromosome isolated from its homolog 
      in somatic cell hybrids. We also studied the same chromosome as an active X in 
      related hybrids. The results provide direct evidence that the expressed XIST 
      locus on the inactive X replicates earlier than its repressed homolog on the 
      active X and earlier than the FRAXA locus which is repressed on this chromosome. 
      The silent XIST locus on the active X replicates late along with F8C which is 
      also not transcribed in these cells. Possible reasons for the different results 
      obtained by Hansen et al. (3) are discussed.
FAU - Torchia, B S
AU  - Torchia BS
AD  - Center for Medical Genetics, The Johns Hopkins University School of Medicine, 
      Baltimore, Maryland 21287-3914, USA.
FAU - Migeon, B R
AU  - Migeon BR
LA  - eng
GR  - HD05465/HD/NICHD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Somat Cell Mol Genet
JT  - Somatic cell and molecular genetics
JID - 8403568
RN  - 0 (Genetic Markers)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Untranslated)
RN  - 0 (Transcription Factors)
RN  - 0 (XIST non-coding RNA)
RN  - 146-91-8 (Guanosine Diphosphate)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Chromosome Mapping
MH  - *DNA Replication
MH  - Fibroblasts
MH  - Genetic Markers
MH  - Genetic Variation
MH  - Guanosine Diphosphate/*genetics
MH  - Humans
MH  - Hybrid Cells
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Mice
MH  - RNA, Long Noncoding
MH  - *RNA, Untranslated
MH  - Skin/cytology/metabolism
MH  - Transcription Factors/*genetics
MH  - *X Chromosome
EDAT- 1995/09/01 00:00
MHDA- 1995/09/01 00:01
CRDT- 1995/09/01 00:00
PHST- 1995/09/01 00:00 [pubmed]
PHST- 1995/09/01 00:01 [medline]
PHST- 1995/09/01 00:00 [entrez]
AID - 10.1007/BF02257467 [doi]
PST - ppublish
SO  - Somat Cell Mol Genet. 1995 Sep;21(5):327-33. doi: 10.1007/BF02257467.