PMID- 8621097
OWN - NLM
STAT- MEDLINE
DCOM- 19960620
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 170
IP  - 1
DP  - 1996 Apr 17
TI  - Cloning and characterization of an IS-like element present in the genome of 
      Brevibacterium lactofermentum ATCC 13869.
PG  - 91-4
AB  - A repetitive DNA element of the Gram+ Brevibacterium lactofermentum (Bl), cloned 
      by a modification of the subtractive hybridization method, contained a 1.4-kb 
      IS-like element, IS13869, which included an open reading frame (ORF) inside a 
      perfect 26-bp terminal inverted repeat (TIR). An 8-bp direct repeat (DR) was 
      found outside each TIR. The ORF encoded a deduced protein of 436 amino acids (49 
      380 Da) with extensive similarity to other known transposases of insertion 
      elements of Mycobacterium smegmatis (IS1096). Pseudomonas sp. (tpnA) and 
      Corynebacterium glutamicum (IS31831). Distinct patterns were observed in 
      different strains of Bl by hybridization with a probe internal to IS13869: four 
      copies of IS13869 occurred in the wild type (wt) and R31 strains, but only three 
      of them were observed in a recA derivative of the wt. Analysis by pulsed-field 
      gel electrophoresis suggested that at least one copy of IS13869 had changed its 
      position inside the chromosome during the lineage of a Bl derivative.
FAU - Correia, A
AU  - Correia A
AD  - Dpt. of Ecology, Genetics and Microbiology, Faculty of Biology, University of 
      Leon, Spain.
FAU - Pisabarro, A
AU  - Pisabarro A
FAU - Castro, J M
AU  - Castro JM
FAU - Martin, J F
AU  - Martin JF
LA  - eng
SI  - GENBANK/M57500
SI  - GENBANK/Z66534
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA Transposable Elements)
RN  - 0 (DNA, Bacterial)
RN  - EC 2.7.7.- (DNA Nucleotidyltransferases)
RN  - EC 2.7.7.- (Transposases)
SB  - IM
MH  - Bacterial Proteins/*genetics
MH  - Base Sequence
MH  - Brevibacterium/*genetics
MH  - Cloning, Molecular
MH  - DNA Nucleotidyltransferases/*genetics
MH  - DNA Transposable Elements/*genetics
MH  - DNA, Bacterial/*genetics
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Repetitive Sequences, Nucleic Acid
MH  - Sequence Alignment
MH  - Sequence Homology, Nucleic Acid
MH  - Species Specificity
MH  - Subtraction Technique
MH  - Transposases
EDAT- 1996/04/17 00:00
MHDA- 1996/04/17 00:01
CRDT- 1996/04/17 00:00
PHST- 1996/04/17 00:00 [pubmed]
PHST- 1996/04/17 00:01 [medline]
PHST- 1996/04/17 00:00 [entrez]
AID - 0378111995008667 [pii]
AID - 10.1016/0378-1119(95)00866-7 [doi]
PST - ppublish
SO  - Gene. 1996 Apr 17;170(1):91-4. doi: 10.1016/0378-1119(95)00866-7.