PMID- 8621657
OWN - NLM
STAT- MEDLINE
DCOM- 19960618
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 271
IP  - 16
DP  - 1996 Apr 19
TI  - Differential expression of the expression site-associated gene I family in 
      African trypanosomes.
PG  - 9771-7
AB  - A minimum of 20 different mRNA species encoding related members of the expression 
      site-associated gene I (ESAG-I) family occur in metacyclic variant antigen type 4 
      bloodstream trypanosomes. None of these ESAG-I mRNAs are derived from the 
      metacyclic variant antigen type 4 variant surface glycoprotein (VSG) gene 
      expression site, and some appear to come from pseudogenes. The ESAG-Is are 
      transcribed in both procyclic and bloodstream trypanosomes, but their mRNAs 
      accumulate to a detectable steady state level only in bloodstream trypanosomes. 
      At least five different groups of 3'-untranslated regions (3'-UTRs) are 
      represented among these ESAG-I mRNAs, suggesting that the 3'-UTR does not 
      contribute to their differential expression. Some ESAG-I mRNAs completely lack a 
      3'-UTR or have only a single nucleotide as a 3'-UTR. Transcription of the ESAG-Is 
      is sensitive to alpha-amanitin, indicating that they are transcribed by a 
      different RNA polymerase than the VSG genes. These results collectively 
      demonstrate that ESAG-I's are a heterogeneous population that can be expressed 
      independently of VSG genes, but like the VSG genes, their mRNAs are present in 
      the bloodstream stage of the parasite and not in the procyclic stage.
FAU - Morgan, R W
AU  - Morgan RW
AD  - Department of Biochemistry, University of Iowa, Iowa City 52242, USA.
FAU - El-Sayed, N M
AU  - El-Sayed NM
FAU - Kepa, J K
AU  - Kepa JK
FAU - Pedram, M
AU  - Pedram M
FAU - Donelson, J E
AU  - Donelson JE
LA  - eng
SI  - GENBANK/M21052
SI  - GENBANK/U40840
SI  - GENBANK/U40841
SI  - GENBANK/U41223
SI  - GENBANK/U41224
SI  - GENBANK/U41225
SI  - GENBANK/U41226
SI  - GENBANK/U41227
SI  - GENBANK/U41228
SI  - GENBANK/U41229
SI  - GENBANK/U41230
SI  - GENBANK/U41231
SI  - GENBANK/U41232
SI  - GENBANK/U41233
SI  - GENBANK/U41234
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Amanitins)
RN  - 0 (ESAG 1 protein, Trypanosoma)
RN  - 0 (Protozoan Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Protozoan)
RN  - 0 (Variant Surface Glycoproteins, Trypanosoma)
SB  - IM
MH  - Amanitins/pharmacology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Northern
MH  - Cell Nucleus/metabolism
MH  - *Gene Expression
MH  - Gene Library
MH  - *Genes, Protozoan
MH  - Molecular Sequence Data
MH  - *Multigene Family
MH  - Protein Biosynthesis
MH  - *Protozoan Proteins
MH  - RNA, Messenger/analysis/biosynthesis
MH  - RNA, Protozoan/analysis/biosynthesis
MH  - Rats
MH  - Sequence Homology, Amino Acid
MH  - Transcription, Genetic/drug effects
MH  - Trypanosoma brucei rhodesiense/*genetics/metabolism
MH  - Variant Surface Glycoproteins, Trypanosoma/*biosynthesis
EDAT- 1996/04/19 00:00
MHDA- 1996/04/19 00:01
CRDT- 1996/04/19 00:00
PHST- 1996/04/19 00:00 [pubmed]
PHST- 1996/04/19 00:01 [medline]
PHST- 1996/04/19 00:00 [entrez]
AID - S0021-9258(17)31401-1 [pii]
AID - 10.1074/jbc.271.16.9771 [doi]
PST - ppublish
SO  - J Biol Chem. 1996 Apr 19;271(16):9771-7. doi: 10.1074/jbc.271.16.9771.