PMID- 8622046
OWN - NLM
STAT- MEDLINE
DCOM- 19960620
LR  - 20170210
IS  - 0732-183X (Print)
IS  - 0732-183X (Linking)
VI  - 14
IP  - 3
DP  - 1996 Mar
TI  - Detection of the t(14;18) chromosomal translocation by interphase cytogenetics 
      with yeast-artificial-chromosome probes in follicular lymphoma and nonneoplastic 
      lymphoproliferation.
PG  - 963-9
AB  - PURPOSE: The aim of this study was to establish a fluorescence in situ 
      hybridization (FISH) technique for the detection of t(14;18)(q32;q21), 
      characteristic for follicular lymphoma (Kiel classification: centroblastic 
      centrocytic [cb-cc] lymphoma). MATERIALS AND METHODS: After the FISH system had 
      been established, parallel studies of lymph node biopsy specimens from 30 
      patients with cb-cc lymphoma and from 32 patients with nonneoplastic 
      lymphoproliferation were performed by means of chromosome analysis, polymerase 
      chain reaction (PCR), and FISH analysis. Two differently labeled 
      yeast-artificial-chromosome (YAC) probes that contained the entire bcl-2 gene and 
      the C-region of the immunoglobulin H (IgH) gene, respectively, were used to 
      detect t(14;18) by FISH. RESULTS: The presence of the translocation is indicated 
      by a red (Cy3)/green (fluorescien isothiocyanate [FITC]) double signal, which 
      corresponds to the IgH/bcl-2 fusion gene, whereas in normal cells the signals are 
      separate. Control studies showed that the double signal is visible in less than 
      1% of normal cells. FISH analysis was able to identify the t(14;18) in all cases 
      of cb-cc lymphoma we studied. All bcl-2 breakpoints can be detected. Combined 
      immunophenotyping and interphase cytogenetics demonstrated that t(14;18) was 
      restricted to CD22+ B lymphocytes and never occurred in CD3+ T lymphocytes. In 
      four of 32 cases of nonneoplastic lymphoproliferation, t(14;18) was also 
      detected. CONCLUSION: FISH turned out to be the most sensitive method to detect 
      t(14;18). Our FISH results confirm PCR data from other groups that found evidence 
      for the presence of t(14;18) in nonneoplastic lymphoproliferation. It needs to be 
      determined whether, in morphologically nonneoplastic processes, t(14;18) is 
      associated with an increased risk for the development of non-Hodgkin's lymphoma.
FAU - Poetsch, M
AU  - Poetsch M
AD  - Department of Human Genetics, University of Kiel, Germany.
FAU - Weber-Matthiesen, K
AU  - Weber-Matthiesen K
FAU - Plendl, H J
AU  - Plendl HJ
FAU - Grote, W
AU  - Grote W
FAU - Schlegelberger, B
AU  - Schlegelberger B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Oncol
JT  - Journal of clinical oncology : official journal of the American Society of 
      Clinical Oncology
JID - 8309333
RN  - 0 (DNA Probes)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - B-Lymphocytes
MH  - Chromosomes, Artificial, Yeast/*genetics
MH  - Chromosomes, Human, Pair 14/*genetics
MH  - Chromosomes, Human, Pair 18/*genetics
MH  - DNA Probes/genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Lymphoma, Follicular/*genetics/pathology
MH  - Lymphoproliferative Disorders/genetics
MH  - Male
MH  - Middle Aged
MH  - Translocation, Genetic/*genetics
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
AID - 10.1200/JCO.1996.14.3.963 [doi]
PST - ppublish
SO  - J Clin Oncol. 1996 Mar;14(3):963-9. doi: 10.1200/JCO.1996.14.3.963.