PMID- 8622129
OWN - NLM
STAT- MEDLINE
DCOM- 19960618
LR  - 20231012
IS  - 0270-6474 (Print)
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 16
IP  - 9
DP  - 1996 May 1
TI  - Retrograde transport of neurotrophins from the eye to the brain in chick embryos: 
      roles of the p75NTR and trkB receptors.
PG  - 2995-3008
AB  - The receptors involved in retrograde transport of neurotrophins from the retina 
      to the isthmo-optic nucleus (ION) of chick embryos were characterized using 
      antibodies to the p75 neurotrophin receptor and trkB receptors. Survival of 
      neurons in the ION has been shown previously to be regulated by target-derived 
      trophic factors with survival promoted or inhibited by ocular injection of 
      brain-derived neurotrophic factor (BDNF) or nerve growth factor (NGF), 
      respectively. In the present paper, we show that during the period of target 
      dependence, these neurons express trkB and p75 neurotrophin receptor but not trkA 
      or trkC mRNAs. We also show that BDNF and NT-3 were transported efficiently at 
      low doses, whereas NGF was transported significantly only at higher doses. The 
      transport of BDNF and NT-3 was reduced by high concentrations of NGF or by 
      antibodies to either trkB or the p75 neurotrophin receptor. Thus both receptors 
      help mediate retrograde transport of these neurotrophins. Ocular injection of the 
      comparatively specific trk inhibitor K252a did not reduce transport of exogenous 
      BDNF, but did induce significant neuronal death in the ION, which could not be 
      prevented by co-injection of BDNF. Thus, transport of BDNF alone does not 
      generate a trophic signal at the cell body when axonal trkB is inactivated. In 
      summary, our results indicate that both p75 neurotrophin and trkB receptors can 
      mediate internalization and retrograde transport of BDNF, but activation of trkB 
      seems to be essential for the survival-promoting actions of this neurotrophin.
FAU - von Bartheld, C S
AU  - von Bartheld CS
AD  - Department of Physiology and Biophysics, University of Washington, Seattle, 
      98195, USA.
FAU - Williams, R
AU  - Williams R
FAU - Lefcort, F
AU  - Lefcort F
FAU - Clary, D O
AU  - Clary DO
FAU - Reichardt, L F
AU  - Reichardt LF
FAU - Bothwell, M
AU  - Bothwell M
LA  - eng
GR  - NS 30305/NS/NINDS NIH HHS/United States
GR  - P01 NS016033-17A10014/NS/NINDS NIH HHS/United States
GR  - HD 29177/HD/NICHD NIH HHS/United States
GR  - P01 NS016033/NS/NINDS NIH HHS/United States
GR  - MH 48200/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Molecular Probes)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurotrophin 3)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor, trkA)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.10.1 (Receptor, trkC)
SB  - IM
MH  - Animals
MH  - Axonal Transport
MH  - Base Sequence
MH  - Biological Transport
MH  - Brain/embryology/*metabolism
MH  - Brain-Derived Neurotrophic Factor
MH  - Chick Embryo
MH  - Eye/embryology/*metabolism
MH  - In Situ Hybridization
MH  - Molecular Probes/genetics
MH  - Molecular Sequence Data
MH  - Nerve Growth Factors/*metabolism
MH  - Nerve Tissue Proteins/metabolism
MH  - Neurons/metabolism
MH  - Neurotrophin 3
MH  - RNA, Messenger/metabolism
MH  - Receptor Protein-Tyrosine Kinases/genetics/*physiology
MH  - Receptor, trkA/genetics
MH  - Receptor, trkB
MH  - Receptor, trkC
MH  - Receptors, Nerve Growth Factor/genetics/*physiology
MH  - Visual Pathways/cytology/embryology/metabolism
PMC - PMC2710111
MID - NIHMS112802
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
PMCR- 1996/11/01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
PHST- 1996/11/01 00:00 [pmc-release]
AID - 10.1523/JNEUROSCI.16-09-02995.1996 [doi]
PST - ppublish
SO  - J Neurosci. 1996 May 1;16(9):2995-3008. doi: 10.1523/JNEUROSCI.16-09-02995.1996.