PMID- 8622597
OWN - NLM
STAT- MEDLINE
DCOM- 19960618
LR  - 20190725
IS  - 0026-0495 (Print)
IS  - 0026-0495 (Linking)
VI  - 45
IP  - 5
DP  - 1996 May
TI  - Lysophosphatidylcholine stimulates the expression and production of MCP-1 by 
      human vascular endothelial cells.
PG  - 559-64
AB  - Lysophosphatidylcholine (LPC increased monocyte chemoattractant protein-1 (MCP-1) 
      messenger RNA concentrations in human umbilical vein endothelial cells (HUVECs). 
      A time-course study showed that the increase in MCP-1 mRNA levels peaked at 6 
      hours after treatment with LPC. The effect of LPC on the accumulation of MCP-I 
      mRNA levels in HUVECs depended on LPC concentration, and the maximal effect was 
      obtained at 50 micromol / L LPC, which induced a sixfold increase in MCP-1 mRNA 
      levels. The amount of MCP-1 released from HUVECs measured using an enzyme-linked 
      immunosorbent assay (ELISA) showed a 38% increase in the presence of 50 
      micromol/L LPC, but not in the presence of phosphatidylcholine or 
      lysophosphatidylethanolamine. Coincubation with staurosporine, a potent inhibitor 
      of protein kinase C (PKC) activity, attenuated the LPC-induced increase in MCP-1 
      mRNA levels by 53%. These results indicate that LPC can induce an increase in 
      MCP-1 mRNA concentrations and stimulate the release of MCP-1 protein from HUVECs, 
      and that the effect of LPC on the MCP-1 gene may be mediated through activation 
      of the PKC pathway.
FAU - Takahara, N
AU  - Takahara N
AD  - Third Department of Medicine, Shiga University of Medical Science, Japan.
FAU - Kashiwagi, A
AU  - Kashiwagi A
FAU - Maegawa, H
AU  - Maegawa H
FAU - Shigeta, Y
AU  - Shigeta Y
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Metabolism
JT  - Metabolism: clinical and experimental
JID - 0375267
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lysophosphatidylcholines)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Chemokine CCL2/biosynthesis/*genetics
MH  - Endothelium, Vascular/cytology/*drug effects/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Kinetics
MH  - Lysophosphatidylcholines/*pharmacology
MH  - Molecular Sequence Data
MH  - Oligodeoxyribonucleotides
MH  - Protein Kinase C/antagonists & inhibitors
MH  - RNA, Messenger/genetics/metabolism
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
AID - S0026-0495(96)90024-4 [pii]
AID - 10.1016/s0026-0495(96)90024-4 [doi]
PST - ppublish
SO  - Metabolism. 1996 May;45(5):559-64. doi: 10.1016/s0026-0495(96)90024-4.