PMID- 8625044
OWN - NLM
STAT- MEDLINE
DCOM- 19960627
LR  - 20190816
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 76
IP  - 10
DP  - 1995 Nov 15
TI  - Flow cytometric DNA analysis and lysosomal cathepsins B and L in locally advanced 
      laryngeal cancer. Relationship with clinicopathologic parameters and prognostic 
      significance.
PG  - 1757-64
AB  - BACKGROUND: The traditional factors of locally advanced laryngeal squamous cell 
      carcinoma (LSCC) have limited predictive value for the identification of high 
      risk patients. Therefore, it is extremely important to define prognostic factors 
      that identify the more aggressive types. Reliable and reproducible prognostic 
      indicators are being investigated to help clinicians identify high risk groups 
      and address more rational treatment. METHODS: Flow cytometric DNA ploidy and 
      S-phase fraction (SPF) measurements were performed on frozen tumor tissues from a 
      consecutive series of 71 patients with Stage III and IV LSCC: Lysosomal cathepsin 
      B and L activity levels were determined biochemically in matched paired sets of 
      tumor tissue and normal mucosa samples. RESULTS: By univariate analysis, lymph 
      node positivity, poor histologic differentiation, DNA aneuploidy, high SPF, and 
      high tumor/mucosa ratio of cathepsin B activity were significantly related to 
      risk of relapse, whereas only DNA aneuploidy and high SPF proved to be 
      significantly related to risk of death. Multivariate analysis showed that high 
      histologic grade and high SPF values (> 15.1%) were independent prognostic 
      factors related to risk of relapse (relative risk [RR] = 3.54; 95% confidence 
      limits [CL] = 1.05-12.0; and RR = 4.22; CL = 1.54-11.6, respectively), whereas 
      only high SPF was related to risk of death (RR = 3.63; CL = 1.17-11.3). 
      CONCLUSIONS: S-phase fraction is an independent predictor of relapse free and 
      overall survival in patients with locally advanced LSCC. On the basis of these 
      findings, SPF should be used in addition to other established prognostic factors 
      to refine the prognostic assessment of these patients further. More studies are 
      needed for a better evaluation of the prognostic significance of DNA ploidy and 
      that of lysosomal cysteine proteinases in these tumors.
FAU - Russo, A
AU  - Russo A
AD  - Institute of Otorhinolaringology B, School of Medicine, University of Palermo, 
      Italy.
FAU - Bazan, V
AU  - Bazan V
FAU - Gebbia, N
AU  - Gebbia N
FAU - Pizzolanti, G
AU  - Pizzolanti G
FAU - Tumminello, F M
AU  - Tumminello FM
FAU - Dardanoni, G
AU  - Dardanoni G
FAU - Ingria, F
AU  - Ingria F
FAU - Restivo, S
AU  - Restivo S
FAU - Tomasino, R M
AU  - Tomasino RM
FAU - Leto, G
AU  - Leto G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
RN  - 0 (DNA, Neoplasm)
RN  - EC 3.4.- (Cathepsins)
RN  - EC 3.4.- (Endopeptidases)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.22.1 (Cathepsin B)
RN  - EC 3.4.22.15 (CTSL protein, human)
RN  - EC 3.4.22.15 (Cathepsin L)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Aneuploidy
MH  - Cathepsin B/*metabolism
MH  - Cathepsin L
MH  - Cathepsins/*metabolism
MH  - Cysteine Endopeptidases
MH  - DNA, Neoplasm/*analysis
MH  - *Endopeptidases
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Laryngeal Neoplasms/enzymology/*genetics/pathology
MH  - Lysosomes/*enzymology
MH  - Male
MH  - Middle Aged
MH  - Prognosis
MH  - S Phase
EDAT- 1995/11/15 00:00
MHDA- 1995/11/15 00:01
CRDT- 1995/11/15 00:00
PHST- 1995/11/15 00:00 [pubmed]
PHST- 1995/11/15 00:01 [medline]
PHST- 1995/11/15 00:00 [entrez]
AID - 10.1002/1097-0142(19951115)76:10<1757::aid-cncr2820761012>3.0.co;2-h [doi]
PST - ppublish
SO  - Cancer. 1995 Nov 15;76(10):1757-64. doi: 
      10.1002/1097-0142(19951115)76:10<1757::aid-cncr2820761012>3.0.co;2-h.