PMID- 8625091
OWN - NLM
STAT- MEDLINE
DCOM- 19960626
LR  - 20190620
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 76
IP  - 2
DP  - 1995 Jul 15
TI  - Flow cytometric identification of cell surface markers on cultured human colonic 
      cell lines using monoclonal antibodies.
PG  - 195-200
AB  - BACKGROUND: The expression of tumor-associated cell surface antigens is a 
      reflection of the state of cell differentiation of tumor cells in culture. 
      METHOD: Monoclonal antibodies (MoAbs) against the tumor-associated antigens 
      carcinoembryonic antigen (CEA) and CA19-9 and the extracellular matrix protein 
      CD44 were used to label the cell surface of human colonic cells in culture. The 
      binding of each antibody to its respective antigen was measured by 
      fluorescence-activated flow cytometry and expressed as a percentage of positive 
      cells. RESULTS: The human colon adenocarcinoma cell (HCAC) line, LS-180, showed 
      strong binding with CEA (81%), CA 19-9 (87%), and CD44 (83%). LS-174t cells, a 
      trypsinized variant of LS-180 cells, showed less binding with CEA (66%) and CA 
      19-9 (49%), but no binding with CD44. With cells from HCAC line HT-29, antigen 
      expression was highly variable for CEA (13% +/- 18) and CD44 (31% +/- 35) but was 
      consistently positive for CA19-9 (33% +/- 13). The expression of CEA in the 
      Caco-2 cell line was weak (24%), whereas there was no expression of CA19-9 and 
      CD44. Normal human colon fibroblast cells (CCD-18Co) did not recognize the 
      monoclonal antibodies to CEA or CA 19-9, but were strongly positive with the CD44 
      antibody (97%). CONCLUSIONS: These results support the concept that the 
      expression of the tumor associated markers CEA and CA19-9 and the cell surface 
      marker CD44 on human colonic cell lines varies with the degree of cellular 
      differentiation. Carcinoembryonic antigen and/or CA19-9 were expressed in all 
      four human colon adenocarcinoma cell lines, but not in the normal colon 
      fibroblast cells (CCD-18Co). Using these two MoAbs appeared to be a more reliable 
      measure of the state of differentiation of human colon adenocarcinoma cells.
FAU - Han, J S
AU  - Han JS
AD  - Nutrient Requirements and Functions Laboratory, Beltsville Human Nutrition 
      Research Center, Agricultural Research Service, United States Department of 
      Agriculture, MD 20705, USA.
FAU - Nair, P P
AU  - Nair PP
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Neoplasm)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Antigens, Surface)
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Antibodies, Neoplasm
MH  - Antigens, Neoplasm/*analysis
MH  - Antigens, Surface/*analysis
MH  - Biomarkers, Tumor/*analysis
MH  - Cell Differentiation
MH  - Colonic Neoplasms/*pathology
MH  - Flow Cytometry/methods
MH  - Fluorescent Antibody Technique, Direct
MH  - Fluorescent Antibody Technique, Indirect
MH  - Humans
MH  - Tumor Cells, Cultured
EDAT- 1995/07/15 00:00
MHDA- 1995/07/15 00:01
CRDT- 1995/07/15 00:00
PHST- 1995/07/15 00:00 [pubmed]
PHST- 1995/07/15 00:01 [medline]
PHST- 1995/07/15 00:00 [entrez]
AID - 10.1002/1097-0142(19950715)76:2<195::aid-cncr2820760206>3.0.co;2-e [doi]
PST - ppublish
SO  - Cancer. 1995 Jul 15;76(2):195-200. doi: 
      10.1002/1097-0142(19950715)76:2<195::aid-cncr2820760206>3.0.co;2-e.