PMID- 8625254
OWN - NLM
STAT- MEDLINE
DCOM- 19960621
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 87
IP  - 2
DP  - 1996 Apr
TI  - High incidence of a second BCR-ABL fusion in chronic myeloid leukemia revealed by 
      interphase cytogenetic analysis on blood and bone marrow smears.
PG  - 107-11
AB  - The t(9;22)(q34;q11) is the single most common chromosomal abnormality in 
      leukemias. Recently, dual-color fluorescence in situ hybridization (FISH) 
      protocols for the detection of the BCR-ABL fusion, which is the molecular 
      counterpart of this translocation, have been described. In the present study, we 
      analyzed blood or bone marrow smears of 46 patients (34 with chronic myeloid 
      leukemia [CML] and 12 with acute lymphoblastic leukemia [ALL]) for the presence 
      of a BCR-ABL fusion. On these clinical routine samples, hybridization was 
      performed with high efficiency and the BCR-ABL fusion was detected reliably. This 
      series includes one case with a Philadelphia chromosome (Ph) on banding analysis 
      and negative reverse transcriptase polymerase chain reaction (RT-PCR) results. 
      Surprisingly, in 13 of the 34 CML patients (4 of 17 patients with chronic phase 
      and 9 of 17 patients with blast crisis), and in 1 of the 12 ALL patients, an 
      additional BCR-ABL fusion was diagnosed in 4% to 72.5% of interphase cells. In 10 
      of these 14 patients, banding data are available; only in two cases was the 
      additional Ph detected by metaphase analysis. The data from this interphase 
      cytogenetic analysis indicate that an additional Ph occurs more frequently than 
      would be assumed based on banding analysis.
FAU - Cabot, G P
AU  - Cabot GP
AD  - Medizinische Klinik und Poliklinik V, Universitat Heidelberg, Germany.
FAU - Bentz, M
AU  - Bentz M
FAU - Scholl, C
AU  - Scholl C
FAU - Moos, M
AU  - Moos M
FAU - Fischer, K
AU  - Fischer K
FAU - Lichter, P
AU  - Lichter P
FAU - Dohner, H
AU  - Dohner H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA Probes)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Bone Marrow/*pathology
MH  - *Chromosome Aberrations
MH  - DNA Probes
MH  - Female
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/blood/*genetics
MH  - Male
MH  - Middle Aged
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
AID - 0165460895002618 [pii]
AID - 10.1016/0165-4608(95)00261-8 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1996 Apr;87(2):107-11. doi: 10.1016/0165-4608(95)00261-8.