PMID- 8625271
OWN - NLM
STAT- MEDLINE
DCOM- 19960621
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 87
IP  - 2
DP  - 1996 Apr
TI  - Fluorescence in situ hybridization-based approaches for detection of 12p 
      overrepresentation, in particular i(12p), in cell lines of human testicular germ 
      cell tumors of adults.
PG  - 95-102
AB  - Overrepresentation of the short arm of chromosome 12 is frequently detected in 
      human testicular germ cell tumors of adolescents and adults (TGCT). This 
      overrepresentation mostly results from the formation of an isochromosome: i(12p). 
      Whether the overrepresentation consistently involves the complete 12p arm 
      including the centromere is still unclear. We studied five TGCT-derived cell 
      lines (NT2, 2102Ep, H12.1, NCCIT, and S2), combining conventional chromosome 
      banding, fluorescence in situ hybridization (FISH), and comparative genomic 
      hybridization (CGH) to investigate the suitability of each of these techniques to 
      detect aberrations involving chromosome 12. Karyotyping showed one or more 
      i(12p)s in NT2, 2102Ep, H12.1, and S2. However, FISH with a centromere-specific 
      probe (p alpha 12H8), a 12p "paint" and a 12p11.2--p12.1 region-specific probe 
      yeast artificial chromosome (YAC)#5 and CGH could not confirm the presence of an 
      i(12p) in S2. Additional randomly distributed 12p sequences were detected by FISH 
      in H12.1, NCCIT, and S2. In most of these cases, (a part of) the centromere was 
      included. All overrepresented 12p regions, except for those in S2, showed 
      hybridization with YAC#5. CGH showed increased copy numbers of the complete 12p 
      arm in the cell lines with one or more i(12p)s but no overrepresentation was 
      noted in the cell lines without i(12p). In metaphase spreads, the centromeric 
      block of the i(12p)s differed in size as compared with those of normal 
      chromosomes 12. This was rarely noted in interphase nuclei. A decrease in size of 
      the centromeric block in 2102Ep and H12.1 caused a weak FISH signal, which was 
      difficult to detect, especially in interphase nuclei. The ratio between p alpha 
      12H8- and YAC#5-derived signals reflected the presence or absence of one or more 
      i(12p)s. Our results indicate that double FISH with a centromere- and a 
      12p-specific probe can be used to detect 12p overrepresentation [including 
      i(12p)] in TGCT both in metaphase spreads and interphase nuclei. CGH confirmed 
      the relative overrepresentation of 12p sequences as detected by FISH and showed 
      that in these cell lines the complete 12p was involved.
FAU - Mostert, M M
AU  - Mostert MM
AD  - Laboratory for Experimental Patho-Oncology Dr. Daniel den Hoed Cancer Center, 
      University Hospital Rotterdam, The Netherlands.
FAU - van de Pol, M
AU  - van de Pol M
FAU - van Echten, J
AU  - van Echten J
FAU - Olde Weghuis, D
AU  - Olde Weghuis D
FAU - Geurts van Kessel, A
AU  - Geurts van Kessel A
FAU - Oosterhuis, J W
AU  - Oosterhuis JW
FAU - Looijenga, L H
AU  - Looijenga LH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adult
MH  - Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 12
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Male
MH  - Neoplasms, Germ Cell and Embryonal/*genetics
MH  - Testicular Neoplasms/*genetics
MH  - Tumor Cells, Cultured
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
AID - 0165460895002332 [pii]
AID - 10.1016/0165-4608(95)00233-2 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1996 Apr;87(2):95-102. doi: 10.1016/0165-4608(95)00233-2.