PMID- 8625556
OWN - NLM
STAT- MEDLINE
DCOM- 19960627
LR  - 20190821
IS  - 0090-1229 (Print)
IS  - 0090-1229 (Linking)
VI  - 78
IP  - 2
DP  - 1996 Feb
TI  - Expression and function of monocyte chemoattractant protein-1 in experimental 
      nephrotic syndrome.
PG  - 140-51
AB  - This study investigates the expression and function of monocyte chemoattractant 
      protein-1 (MCP-1) in rats with aminonucleoside nephrosis induced by a single 
      intraperitoneal injection of puromycin aminonucleoside (PAN). On Day 7, 
      PAN-treated rats had a sixfold increase in renal MCP-1 messenger (m)RNA levels 
      and a twofold increase in interleukin-1 beta mRNA levels. During the course of 
      PAN nephrosis, most of the de novo MCP-1 protein resembled protein droplets that 
      were prominent in glomeruli between Days 3 and 14 and weaker but visible in 
      tubules between Days 5 and 10. In addition, occasional tubules showed a 
      cytoplasmic staining pattern for MCP-1. Two studies evaluated the effect of MCP-1 
      neutralization on renal monocyte recruitment. In the first study, PAN-treated 
      rats were treated with affinity-purified MCP-1-neutralizing rabbit IgG on Days 0, 
      1, 3, and 5; kidneys were harvested on Day 7. There was no difference in the mean 
      number of interstitial macrophages [119 +/- 28 vs 88 +/- 9 ED-1+ cells/1000 
      tubulointerstitial (TI) cells; 106 +/- 28 vs 119 +/- 33 Ia+ cells/1000 TI cells] 
      or intraglomerular macrophages [2.0 +/- 0.9 vs 1.7 +/- 0.5 ED-1+ cells/glomerular 
      cross section (gcs); 1.2 +/- 0.3 vs 1.1 +/- 0.4 Ia+ cells/gcs] compared with 
      nephrotic rats treated with nonimmune rabbit IgG. In the second study, a group of 
      PAN-treated rats was treated with MCP-1-neutralizing IgG administered 
      continuously by an intraperitoneal miniosmotic pump for 7 days and was compared 
      with a control group treated in an identical fashion with PAN and nonimmune IgG. 
      On Day 7 there was no difference in the mean number of interstitial macrophages 
      (55 +/- 45 vs 67 +/- 16 ED-1+ and 70 +/- 63 vs 61 +/- 13 Ia+ cells/1000 TI cells) 
      and intraglomerular macrophages (1.0 +/- 0.4 vs 1.6 +/- 0.9 ED-1+ and 0.6 +/- 0.1 
      vs 1.1 +/- 0.7 Ia+ cells/gcs). The results of this study suggest that although 
      MCP-1 gene and protein expression are increased in the kidneys of rats with 
      aminonucleoside nephrosis, MCP-1 does not appear to play an essential role in 
      early renal monocyte recruitment in this model.
FAU - Eddy, A A
AU  - Eddy AA
AD  - Department of Paediatrics, The Hospital for Sick Children, University of Toronto, 
      Ontario, Canada.
FAU - Warren, J S
AU  - Warren JS
LA  - eng
GR  - HL40526/HL/NHLBI NIH HHS/United States
GR  - HL48287/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Clin Immunol Immunopathol
JT  - Clinical immunology and immunopathology
JID - 0356637
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Immune Sera)
RN  - 0 (Immunoglobulin G)
RN  - 58-60-6 (Puromycin Aminonucleoside)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis/immunology/*physiology
MH  - Chemotaxis, Leukocyte/*immunology
MH  - Cytokines/biosynthesis
MH  - Female
MH  - Humans
MH  - Immune Sera/administration & dosage
MH  - Immunoglobulin G/metabolism
MH  - Injections, Intraperitoneal
MH  - Injections, Intravenous
MH  - Monocytes/*immunology
MH  - Nephrotic Syndrome/chemically induced/*immunology
MH  - Puromycin Aminonucleoside
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Rats, Sprague-Dawley
MH  - Time Factors
EDAT- 1996/02/01 00:00
MHDA- 1996/02/01 00:01
CRDT- 1996/02/01 00:00
PHST- 1996/02/01 00:00 [pubmed]
PHST- 1996/02/01 00:01 [medline]
PHST- 1996/02/01 00:00 [entrez]
AID - S0090122996900239 [pii]
AID - 10.1006/clin.1996.0023 [doi]
PST - ppublish
SO  - Clin Immunol Immunopathol. 1996 Feb;78(2):140-51. doi: 10.1006/clin.1996.0023.