PMID- 8625558
OWN - NLM
STAT- MEDLINE
DCOM- 19960627
LR  - 20220318
IS  - 0090-1229 (Print)
IS  - 0090-1229 (Linking)
VI  - 78
IP  - 2
DP  - 1996 Feb
TI  - Markedly elevated serum MMP-9 (gelatinase B) levels in rheumatoid arthritis: a 
      potentially useful laboratory marker.
PG  - 161-71
AB  - In an attempt to find a potentially useful serum marker in rheumatoid arthritis 
      (RA) which reflects underlying pathogenic mechanisms, we measured the circulating 
      levels of matrix-degrading metalloproteinase-9 (MMP-9), also termed gelatinase B, 
      in sera and synovial fluid (SF) from patients with RA and also quantitated the 
      deposition and local synthesis of MMP-9 in RA synovium. Clinical samples, 
      subjected to gelatin substrate zymography, antigenic immunoassay, and a 
      quantitative substrate degradation assay, revealed elevated 92- and 72-kDa 
      proenzyme forms of MMP-9 and MMP-2 in RA sera and SF compared with healthy 
      controls. Immunostaining on fresh RA synovial specimens revealed MMP-9 within 
      vascular walls in fibroblast-like cells and macrophages; mRNA synthesis was 
      detected using reverse transcriptase in situ PCR. In summary, MMP-9 levels are 
      substantially elevated in the sera and SF from patients with RA. The RA synovium 
      is a source of this MMP-9 production, with abundant mRNA and protein observed 
      within several different type of rheumatoid synovial cells.
FAU - Gruber, B L
AU  - Gruber BL
AD  - Department of Medicine, State University of New York, Stony Brook 11794-8161, 
      USA.
FAU - Sorbi, D
AU  - Sorbi D
FAU - French, D L
AU  - French DL
FAU - Marchese, M J
AU  - Marchese MJ
FAU - Nuovo, G J
AU  - Nuovo GJ
FAU - Kew, R R
AU  - Kew RR
FAU - Arbeit, L A
AU  - Arbeit LA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Clin Immunol Immunopathol
JT  - Clinical immunology and immunopathology
JID - 0356637
RN  - 0 (Antigens)
RN  - 0 (Biomarkers)
RN  - EC 3.4.24.- (Collagenases)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Antigens/analysis
MH  - Arthritis, Rheumatoid/blood/*diagnosis/enzymology
MH  - Biomarkers/*blood
MH  - Collagenases/*blood/immunology/metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Matrix Metalloproteinase 9
MH  - Polymerase Chain Reaction
MH  - Substrate Specificity
MH  - Synovial Fluid/enzymology
EDAT- 1996/02/01 00:00
MHDA- 1996/02/01 00:01
CRDT- 1996/02/01 00:00
PHST- 1996/02/01 00:00 [pubmed]
PHST- 1996/02/01 00:01 [medline]
PHST- 1996/02/01 00:00 [entrez]
AID - S0090122996900252 [pii]
AID - 10.1006/clin.1996.0025 [doi]
PST - ppublish
SO  - Clin Immunol Immunopathol. 1996 Feb;78(2):161-71. doi: 10.1006/clin.1996.0025.