PMID- 8627124
OWN - NLM
STAT- MEDLINE
DCOM- 19960624
LR  - 20190909
IS  - 0955-3002 (Print)
IS  - 0955-3002 (Linking)
VI  - 69
IP  - 4
DP  - 1996 Apr
TI  - Classification of X-ray-induced Robertsonian fusion-like configurations in mouse 
      splenocytes.
PG  - 421-7
AB  - A characteristic karyotypic feature of mouse chromosomes is the presence of large 
      blocks of heterochromatin in the vicinity of the centromeres. Breakage inside 
      this centromeric heterochromatin might result in the formation of abnormal 
      chromosomes, very similar to the metacentric chromosomes derived from 
      Robertsonian fusion. X-rays are very efficient in inducing these Robertsonian 
      fusion-like configurations (RLC) in cultured mouse splenocytes. Observed 
      frequencies of these RLC increase in a linear-quadratic manner with dose. Two 
      types of RLC were found. The first type (approximately 70% of induced RLC) has 
      heterochromatin only in the middle of the chromosome and appears as a metacentric 
      chromosome, whereas the other type (approximately 30%) has a heterochromatic 
      block inside the chromosome arm (and has the appearance of a dicentric 
      chromosome). Induced RLC are difficult to classify as either a stable or unstable 
      aberration, based only on traditional cytogenetic techniques such as C-banding. 
      Here we describe a cytogenetic approach to achieving better insight into the 
      molecular organization of RLC. We utilized two-colour fluorescence in situ 
      hybridization (FISH) using a combination of mouse minor satellite DNA probe and 
      telomeric probe. Over 90% of RLC did not have detectable minor satellite arrays 
      inside the interstitial heterochromatin. Consequently, most of the RLC of the 
      first type should be classified as acentric fragments and those of the second 
      type as translocations. The chance of inducing true Robertsonian fusions in mouse 
      splenocytes by X-rays is < 2.5% based on the total RLC observed.
FAU - Boei, J J
AU  - Boei JJ
AD  - Department of Radiation Genetics and Chemical Mutagenesis, Leiden University, The 
      Netherlands.
FAU - Natarajan, A T
AU  - Natarajan AT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Centromere/*radiation effects
MH  - *Chromosome Aberrations
MH  - Female
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Spleen/*radiation effects/ultrastructure
MH  - X-Rays
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
AID - 10.1080/095530096145715 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 1996 Apr;69(4):421-7. doi: 10.1080/095530096145715.