PMID- 8627126
OWN - NLM
STAT- MEDLINE
DCOM- 19960624
LR  - 20190909
IS  - 0955-3002 (Print)
IS  - 0955-3002 (Linking)
VI  - 69
IP  - 4
DP  - 1996 Apr
TI  - Induction and persistence of cytogenetic damage in mouse splenocytes following 
      whole-body X-irradiation analysed by fluorescence in situ hybridization. I. 
      Dicentrics and translocations.
PG  - 437-46
AB  - Chromosome translocations (stable aberrations) can persist longer during cellular 
      proliferation than dicentrics (unstable aberrations). It is important to know the 
      kinetics of the elimination of dicentrics and to what extent translocations 
      persist in an in vivo cell population after irradiation. The female Swiss mouse 
      were used to study the induction and persistence of dicentrics and translocations 
      in splenocytes up to 112 days after exposure to 2 Gy whole-body X-irradiation. 
      Metaphase spreads at different time intervals were analyzed by fluorescence in 
      situ hybridization (FISH) using chromosome-specific DNA libraries for chromosomes 
      1, 11 and 13. The frequencies of dicentrics and translocations appear to be equal 
      immediately after irradiation. Frequencies of dicentrics decreased exponentially 
      with time according to the relationship D = ae(-kt). The rate of elimination was 
      faster in the early period (days 0-14) than in the later period (> or = 14 days). 
      The frequency of translocations was constant in the period 0-7 days and then 
      decreased linearly or exponentially. For the whole period, the trend is highly 
      significant. As mouse chromosome painting probes are becoming available and by 
      using FISH, an in vivo mouse model for the analysis of translocations has become 
      feasible. As translocations are involved in carcinogenesis and genetic disorders, 
      risk estimation for induction of translocations by ionizing radiation can be made 
      with greater confidence and extrapolated to the human situation.
FAU - Hande, M P
AU  - Hande MP
AD  - Department of Radiation Genetics and Chemical Mutagenesis, Leiden University, The 
      Netherlands.
FAU - Boei, J J
AU  - Boei JJ
FAU - Granath, F
AU  - Granath F
FAU - Natarajan, A T
AU  - Natarajan AT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Spleen/*radiation effects/ultrastructure
MH  - *Translocation, Genetic
MH  - Whole-Body Irradiation
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
AID - 10.1080/095530096145733 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 1996 Apr;69(4):437-46. doi: 10.1080/095530096145733.