PMID- 8627230
OWN - NLM
STAT- MEDLINE
DCOM- 19960627
LR  - 20061115
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 77 ( Pt 2 )
DP  - 1996 Feb
TI  - Recognition of the initiation codon for protein synthesis in foot-and-mouth 
      disease virus RNA.
PG  - 265-72
AB  - Foot-and-mouth disease virus (FMDV) RNA utilizes two in-frame initiation codons 
      to produce two precursor proteins with identical carboxy termini. The 5' 
      untranslated region (5'UTR) directs the ribosome to internal sequences without 
      the need for a cap structure as used in host mRNAs. The FMDV 5'UTR was cloned 
      upstream of the reporter gene chloramphenicol acetyltransferase (CAT) in order to 
      study the selection of initiation site and to facilitate quantification of the 
      translation products. After in vitro transcription with T7 RNA polymerase and 
      translation in rabbit reticulocyte lysate, the two CAT products, resulting from 
      initiation from the two initiation codons, were quantified. The downstream 
      initiator AUG (AUGLb) was selected more efficiently in the wild-type 5'UTR. In 
      truncated RNA, the upstream initiation site (AUGLab) was more efficiently 
      utilized than in the wild-type 5'UTR. Protein synthesis initiation factors were 
      added to translation assays to determine whether these factors influenced 
      initiation site selection. Addition of eIF-2 and of eIF-2B changed the selection 
      process for both types of RNA. These factors induced a 2.5-fold higher usage of 
      the upstream AUGLab for wild-type and 5'UTR-truncated RNA. A change in mRNA 
      concentration also induced a change in the usage of initiation codons; however, 
      the effect of eIF-2 was measured over a broad range of mRNA concentrations. In 
      conclusion, eIF-2 mediates the recognition of the initiation codon during both 
      cap-dependent and internal ribosome entry site-dependent initiation.
FAU - Thomas, A A
AU  - Thomas AA
AD  - Department of Molecular Cell Biology, Utrecht University, The Netherlands. 
      a.a.m.thomas/biol.ruu.nl
FAU - Rijnbrand, R
AU  - Rijnbrand R
FAU - Voorma, H O
AU  - Voorma HO
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Codon)
RN  - 0 (Eukaryotic Initiation Factor-2)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Aphthovirus/*genetics
MH  - *Codon
MH  - Eukaryotic Initiation Factor-2/physiology
MH  - RNA, Viral/*metabolism
MH  - Rabbits
MH  - Ribosomes/metabolism
MH  - Viral Proteins/*biosynthesis
EDAT- 1996/02/01 00:00
MHDA- 1996/02/01 00:01
CRDT- 1996/02/01 00:00
PHST- 1996/02/01 00:00 [pubmed]
PHST- 1996/02/01 00:01 [medline]
PHST- 1996/02/01 00:00 [entrez]
AID - 10.1099/0022-1317-77-2-265 [doi]
PST - ppublish
SO  - J Gen Virol. 1996 Feb;77 ( Pt 2 ):265-72. doi: 10.1099/0022-1317-77-2-265.