PMID- 8627363
OWN - NLM
STAT- MEDLINE
DCOM- 19960621
LR  - 20191101
IS  - 0270-6474 (Print)
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 16
IP  - 10
DP  - 1996 May 15
TI  - Synaptic modulation by neurotrophic factors: differential and synergistic effects 
      of brain-derived neurotrophic factor and ciliary neurotrophic factor.
PG  - 3256-64
AB  - Extracellular application of brain-derived neurotrophic factor (BDNF) and ciliary 
      neurotrophic factor (CNTF) to developing neuromuscular junctions in Xenopus 
      nerve-muscle cultures resulted in an increase in the frequency of spontaneous 
      synaptic currents (SSCs) and in the amplitude of nerve-evoked synaptic currents. 
      Analyses of the amplitude and time course of the SSCs suggest that these effects 
      are attributable to elevation of presynaptic transmitter release. The actions of 
      these two factors on the transmitter secretion process, however, are distinctly 
      different. Fura-2 Ca2+ imaging showed that an increase in presynaptic cytosolic 
      Ca2+ ([Ca2+]i) accompanied the synaptic potentiation by BDNF, whereas no change 
      in [Ca2+]i was observed during synaptic potentiation by CNTF. Removing external 
      Ca2+ also abolished the potentiating effect of BDNF but did not influence the 
      CNTF effect. Moreover, the two factors exerted different effects on the 
      short-term synaptic plasticity. Paired-pulse facilitation normally found at these 
      synapses was reduced by BDNF but unaffected by CNTF; CNTF, but not BDNF, reduced 
      the extent of synaptic depression during high-frequency tetanic stimulation. 
      Finally, the potentiation effect of BDNF and CNTF on spontaneous transmitter 
      release was additive when both factors were applied together to the synapse at 
      saturating concentrations (100 ng/ml) and was highly synergistic when low doses 
      (1 and 10 ng/ml) of both factors were used. These results suggest that because of 
      their differential effects on the secretory machinery, BDNF and CNTF may act 
      cooperatively in modulating the development and functioning of synapses.
FAU - Stoop, R
AU  - Stoop R
AD  - Department of Biological Sciences, Columbia University, New York, New York 10027, 
      USA.
FAU - Poo, M M
AU  - Poo MM
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Ciliary Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor
MH  - Cells, Cultured
MH  - Ciliary Neurotrophic Factor
MH  - Nerve Growth Factors/*pharmacology
MH  - Nerve Tissue Proteins/*pharmacology
MH  - Neuromuscular Junction/*drug effects
MH  - Synapses/*drug effects/physiology
MH  - Time Factors
MH  - Xenopus
PMC - PMC6579145
EDAT- 1996/05/15 00:00
MHDA- 1996/05/15 00:01
PMCR- 1996/11/15
CRDT- 1996/05/15 00:00
PHST- 1996/05/15 00:00 [pubmed]
PHST- 1996/05/15 00:01 [medline]
PHST- 1996/05/15 00:00 [entrez]
PHST- 1996/11/15 00:00 [pmc-release]
AID - 10.1523/JNEUROSCI.16-10-03256.1996 [doi]
PST - ppublish
SO  - J Neurosci. 1996 May 15;16(10):3256-64. doi: 10.1523/JNEUROSCI.16-10-03256.1996.