PMID- 8627701
OWN - NLM
STAT- MEDLINE
DCOM- 19960627
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 70
IP  - 3
DP  - 1996 Mar
TI  - Cyclic AMP-responsive element-dependent activation of Epstein-Barr virus zebra 
      promoter by human herpesvirus 6.
PG  - 1784-91
AB  - We have recently shown that infection of Epstein-Barr virus (EBV) genome-positive 
      B cells by human herpesvirus 6 (HHV-6) results in the expression of the 
      immediate-early EBV Zebra gene, followed by virus replication (L. Flamand, I. 
      Stefanescu, D. V. Ablashi, and J. Menezes, J. Virol. 67:6768-6777, 1993). Here we 
      show that HHV-6 upregulates Zebra gene transcription through a cyclic 
      AMP-responsive element (CRE) located within the Zebra promoter (Zp). Using human 
      B- or T-cell lines transfected with ZpCat reporter gene constructs, we 
      demonstrate that a region designated the ZII domain of Zp is the target of HHV-6 
      transactivation. Mutation of the consensus AP-1/CRE site within ZII abolished the 
      inducibility of Zp by HHV-6, whereas positioning of the ZII domain upstream of 
      the beta-globin minimal promoter conferred responsiveness following HHV-6 
      infection. Binding of these factors to ZII was prevented by oligonucleotides 
      containing CRE but not by AP-1 consensus sequences. Antibodies against 
      CRE-binding (CREB) protein but not against c-Fos or c-Jun were able to supershift 
      the DNA-protein complex, identifying the nature of the transcription factor which 
      binds to ZII as a member of the CREB family of proteins. Finally, transfection of 
      CREB protein and protein kinase A expression vectors were found to activate Zp in 
      Jurkat cells, suggesting that phosphorylated form of CREB protein can play a 
      determining role in the EBV reactivation process.
FAU - Flamand, L
AU  - Flamand L
AD  - Laboratory of Immunovirology, University of Montreal, Quebec, Canada.
FAU - Menezes, J
AU  - Menezes J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (BZLF1 protein, Herpesvirus 4, Human)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (DNA, Viral)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nucleic Acid Synthesis Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factor AP-1)
RN  - 0 (Viral Proteins)
RN  - N919E46723 (Phosphonoacetic Acid)
SB  - IM
MH  - B-Lymphocytes/cytology/virology
MH  - Base Sequence
MH  - Cell Line
MH  - Cyclic AMP Response Element-Binding Protein/*metabolism
MH  - DNA, Viral
MH  - DNA-Binding Proteins/*genetics/metabolism
MH  - Gene Expression Regulation, Viral
MH  - Herpesvirus 6, Human/*physiology
MH  - Humans
MH  - Kinetics
MH  - Molecular Sequence Data
MH  - Nucleic Acid Synthesis Inhibitors
MH  - Phosphonoacetic Acid/pharmacology
MH  - Phosphorylation
MH  - *Promoter Regions, Genetic
MH  - RNA, Messenger
MH  - RNA, Viral
MH  - T-Lymphocytes/cytology/virology
MH  - Trans-Activators/*genetics/metabolism
MH  - Transcription Factor AP-1/metabolism
MH  - *Transcriptional Activation
MH  - *Viral Proteins
PMC - PMC190004
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
PMCR- 1996/03/01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
PHST- 1996/03/01 00:00 [pmc-release]
AID - 10.1128/JVI.70.3.1784-1791.1996 [doi]
PST - ppublish
SO  - J Virol. 1996 Mar;70(3):1784-91. doi: 10.1128/JVI.70.3.1784-1791.1996.