PMID- 8627779
OWN - NLM
STAT- MEDLINE
DCOM- 19960621
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 70
IP  - 5
DP  - 1996 May
TI  - In vivo effects of a recombinant vaccinia virus expressing a mouse mammary tumor 
      virus superantigen.
PG  - 3026-31
AB  - Early after infection, the mouse mammary tumor virus (MMTV) expresses a 
      superantigen (SAg) at the surface of B lymphocytes. Interaction with the T-cell 
      receptor Vbeta domain induces a polyclonal proliferative response of the 
      SAg-reactive T cells. Stimulated T cells become anergic and are deleted from the 
      T-cell repertoire. We have used a recombinant vaccinia virus encoding the 
      MMTV(GR) SAg to dissect the effects of the retroviral SAg during an unrelated 
      viral infection. Subcutaneous infection with this recombinant vaccinia virus 
      induces a very rapid increase of Vbeta14 T cells in the draining lymph node. This 
      stimulation does not require a large Plumber of infectious particles and is not 
      strictly dependent on the expression of the major histocompatibility complex 
      class II I-E molecule, as it is required after MMTV(GR) infection. In contrast to 
      MMTV infection during which B cells are infected, we do not observe any clonal 
      deletion of the reactive T cells following the initial stimulation phase. Our 
      data show that contrary to the case with MMTV, macrophages but not B cells are 
      the targets of infection by vaccinia virus in the lymph node, indicating the 
      ability of these cells to present a retroviral SAg. The altered SAg expression in 
      a different target cell observed during recombinant vaccinia virus infection 
      therefore results in significant changes in the SAg response.
FAU - Krummenacher, C
AU  - Krummenacher C
AD  - Institute for Microbiology, University of Lausanne, Switzerland.
FAU - Diggelmann, H
AU  - Diggelmann H
FAU - Acha-Orbea, H
AU  - Acha-Orbea H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antigens, Viral)
RN  - 0 (DNA Primers)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
RN  - 0 (Superantigens)
SB  - IM
MH  - Animals
MH  - Antigens, Viral/biosynthesis/*immunology
MH  - Base Sequence
MH  - DNA Primers
MH  - Flow Cytometry
MH  - Gene Expression
MH  - Immunity, Cellular
MH  - Lymph Nodes/immunology/virology
MH  - Mammary Tumor Virus, Mouse/immunology/metabolism/*physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Oligonucleotide Probes
MH  - Polymerase Chain Reaction
MH  - Receptors, Antigen, T-Cell, alpha-beta/*immunology
MH  - Recombination, Genetic
MH  - Superantigens/biosynthesis/*immunology
MH  - T-Lymphocytes/*immunology/virology
MH  - Time Factors
MH  - Vaccinia/*immunology
MH  - Vaccinia virus/metabolism/*physiology
MH  - Viral Plaque Assay
MH  - Virus Replication
PMC - PMC190162
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
PMCR- 1996/05/01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
PHST- 1996/05/01 00:00 [pmc-release]
AID - 10.1128/JVI.70.5.3026-3031.1996 [doi]
PST - ppublish
SO  - J Virol. 1996 May;70(5):3026-31. doi: 10.1128/JVI.70.5.3026-3031.1996.