PMID- 8629392
OWN - NLM
STAT- MEDLINE
DCOM- 19960621
LR  - 20180217
IS  - 0001-5547 (Print)
IS  - 0001-5547 (Linking)
VI  - 40
IP  - 2
DP  - 1996 Mar-Apr
TI  - Fluorescence in situ hybridization for detecting erbB-2 amplification in breast 
      tumor fine needle aspiration biopsies.
PG  - 164-73
AB  - OBJECTIVE: To evaluate the feasibility of erbB-2 amplification analysis of fine 
      needle aspiration (FNA) biopsies. STUDY DESIGN: FNA smears and dissociated nuclei 
      from 58 breast cancer samples were examined by dual-labeling fluorescence in situ 
      hybridization (FISH) with probes for centromere 17 and the erbB-2 gene. The 
      results were compared with the outcome of erbB-2 immunohistochemistry. RESULTS: 
      Tumors were categorized according to the erbB-2/centromere 17 signal ratio. There 
      were 23 tumors with high-level amplification, four cases with a low-level erbB-2 
      gain and 27 tumors with normal erbB-2 content. Four tumors showed an erbB-2 
      deletion, all in patients < or = 42 years of age. ErbB-2 amplification was 
      strongly associated with positive erbB-2 immunostaining (P < .0001). Comparison 
      of FISH analysis on dissociated cells and on FNA biopsies showed high 
      correspondence (P < .0001). CONCLUSION: FISH allows reliable detection of erbB-2 
      gene amplification on FNA biopsies.
FAU - Sauter, G
AU  - Sauter G
AD  - Department of Pathology, University of Basel, Switzerland.
FAU - Feichter, G
AU  - Feichter G
FAU - Torhorst, J
AU  - Torhorst J
FAU - Moch, H
AU  - Moch H
FAU - Novotna, H
AU  - Novotna H
FAU - Wagner, U
AU  - Wagner U
FAU - Durmuller, U
AU  - Durmuller U
FAU - Waldman, F M
AU  - Waldman FM
LA  - eng
GR  - CA 44768/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Acta Cytol
JT  - Acta cytologica
JID - 0370307
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biopsy, Needle
MH  - Breast Neoplasms/*genetics/pathology
MH  - Carcinoma in Situ/genetics/pathology
MH  - Carcinoma, Ductal, Breast/genetics/pathology
MH  - Carcinoma, Lobular/genetics/pathology
MH  - Carcinoma, Medullary/genetics/pathology
MH  - Centromere
MH  - Evaluation Studies as Topic
MH  - Feasibility Studies
MH  - Female
MH  - *Gene Amplification
MH  - Gene Dosage
MH  - *Genes, erbB-2
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Middle Aged
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
AID - 10.1159/000333683 [doi]
PST - ppublish
SO  - Acta Cytol. 1996 Mar-Apr;40(2):164-73. doi: 10.1159/000333683.