PMID- 8629622
OWN - NLM
STAT- MEDLINE
DCOM- 19960625
LR  - 20190815
IS  - 0272-6386 (Print)
IS  - 0272-6386 (Linking)
VI  - 27
IP  - 5
DP  - 1996 May
TI  - Monocyte chemoattractant protein-1 levels in patients with glomerular disease.
PG  - 640-6
AB  - Chemokines such as monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 
      have been implicated in recruiting leukocytes to the glomerulus during immune 
      renal injury. To detect the expression of MCP-1 in human glomerular disease, we 
      measured urinary MCP-1 levels in patients with a variety of glomerulopathies. 
      These data demonstrated that MCP-1 was present in the urine of patients with 
      glomerular disease and that patients with inflammatory glomerulopathies had 
      higher levels of urinary MCP-1. Urinary MCP-1 correlated with the extent of 
      proteinuria (r = 0.71, P < 0.0001), but not with serum MCP-1 levels (r = 0.14, P 
      > 0.3). The MCP-1 present in urine was biologically active, increasing monocyte 
      migration in a microchemotaxis assay. This activity was attenuated by the 
      addition of anti-MCP-1 antibody to the samples. Enumerating glomerular 
      macrophages demonstrated that glomerular inflammation was correlated with urinary 
      MCP-1 levels (r = 0.53, P < 0.02). Individuals with light microscopic evidence of 
      severe glomerular injury (ie, crescents, necrosis, endocapillary proliferation) 
      had significantly higher levels of MCP-1 in their urine than patients with less 
      severe glomerular changes (1,962 +/- 612 pg MCP-1/mg creatinine v 314 +/- 45 pg 
      MCP-1/mg creatinine; P < 0.002). Taken together, these results suggest that 
      urinary MCP-1 reflects the extent to which MCP-1 that is produced in the 
      glomerulus participates in the glomerular inflammatory response.
FAU - Rovin, B H
AU  - Rovin BH
AD  - Department of Medicine, Ohio State University, Columbus, 43210, USA.
FAU - Doe, N
AU  - Doe N
FAU - Tan, L C
AU  - Tan LC
LA  - eng
GR  - R29 DK46055/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Kidney Dis
JT  - American journal of kidney diseases : the official journal of the National Kidney 
      Foundation
JID - 8110075
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
RN  - AYI8EX34EU (Creatinine)
SB  - IM
MH  - Chemokine CCL2/blood/*urine
MH  - Chemotaxis, Leukocyte
MH  - Creatinine/urine
MH  - Female
MH  - Glomerulonephritis/blood/pathology/*urine
MH  - Humans
MH  - Interleukin-8/urine
MH  - Kidney Glomerulus/immunology/pathology
MH  - Macrophage Activation
MH  - Macrophages/pathology
MH  - Male
MH  - Necrosis
MH  - Proteinuria/urine
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
AID - S0272-6386(96)90097-9 [pii]
AID - 10.1016/s0272-6386(96)90097-9 [doi]
PST - ppublish
SO  - Am J Kidney Dis. 1996 May;27(5):640-6. doi: 10.1016/s0272-6386(96)90097-9.