PMID- 8630983
OWN - NLM
STAT- MEDLINE
DCOM- 19960701
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 88
IP  - 1
DP  - 1996 May
TI  - The use of FISH with chromosome-specific repetitive DNA probes for the follow-up 
      of leukemia patients. Correlations and discrepancies with bone marrow cytology.
PG  - 69-75
AB  - The use of fluorescence in situ hybridization (FISH) for the purpose of repeated 
      follow-up examination of bone marrow samples from 38 leukemia patients was 
      investigated. On the basis of conventional cytogenetic analysis, patients with 
      acute leukemia whose leukemic cells carried numerical chromosomal aberrations 
      were selected and followed with repetitive DNA probes that specifically hybridize 
      to one chromosome type. Repeated cytogenetic metaphase analyses would have been 
      laborious and not sensitive or quantitative enough to follow declining numbers of 
      aberrant cells. FISH, as an interphase cytogenetic technique, provides a rapid 
      and simple alternative with high sensitivity. Although FISH data before and after 
      chemotherapy were in agreement with bone marrow cytology in 30 of 38 patients, 
      discrepancies were noticed in specific cases. These could be explained by the 
      presence of cytogenetically distinct subclones that behave differently during 
      treatment, the presence of differentiated leukemic cells, changes in the 
      chromosomal constitution caused by clonal relapse, or the fact that a numerical 
      aberration is found by conventional chromosome banding analysis while the target 
      region to which the probe is directed is still present in the nucleus as a 
      diploid set.
FAU - Arkesteijn, G J
AU  - Arkesteijn GJ
AD  - Institute of Hematology, Erasmus University, Rotterdam, The Netherlands.
FAU - Erpelinck, S L
AU  - Erpelinck SL
FAU - Martens, A C
AU  - Martens AC
FAU - Hagemeijer, A
AU  - Hagemeijer A
FAU - Hagenbeek, A
AU  - Hagenbeek A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA Probes)
SB  - IM
MH  - Bone Marrow/*pathology
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 17
MH  - *DNA Probes
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Leukemia/drug therapy/*genetics/pathology
MH  - Leukemia, Myeloid, Acute/drug therapy/genetics/pathology
MH  - Male
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/drug therapy/genetics/pathology
MH  - Remission Induction
MH  - *Repetitive Sequences, Nucleic Acid
MH  - X Chromosome
MH  - Y Chromosome
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
AID - 0165460895002782 [pii]
AID - 10.1016/0165-4608(95)00278-2 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1996 May;88(1):69-75. doi: 10.1016/0165-4608(95)00278-2.