PMID- 8630985
OWN - NLM
STAT- MEDLINE
DCOM- 19960701
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 88
IP  - 1
DP  - 1996 May
TI  - Detection of genetic alterations in micrometastatic cells in bone marrow of 
      cancer patients by fluorescence in situ hybridization.
PG  - 8-16
AB  - Detection of micrometastatic tumor cells in bone marrow of cancer patients has 
      been shown to be of prognostic significance. To further characterize these cells, 
      we combined antibody labeling and fluorescence in situ hybridization (FISH). For 
      detection of numerical changes of chromosome 17, nine patients with proven breast 
      cancer whose bone marrow contained epithelial tumor cells were evaluated. 
      Epithelial cells were stained by anticytokeratin antibody. Afterwards FISH was 
      performed using an alpha-satellite probe specific for chromosome 17. In a second 
      series bone marrow epithelial cells of eight patients with breast cancer and of 
      six with prostatic cancer were evaluated for the amplification of HER-2/neu by 
      using a gene-specific DNA probe. In the first series four patients had only 
      single epithelial cells in their bone marrow. Only one single cell showed five 
      hybridization signals, whereas all other single cells showed two or less. Five 
      patients had clusters of epithelial cells in bone marrow with or without 
      additional single cells. One hundred four cells had three or more hybridization 
      signals and 103 of these polysomic cells were located in tumor cell clusters. In 
      the second series we could detect HER-2/neu amplification in bone marrow 
      epithelial tumor cells in two of eight patients with breast cancer but in none of 
      the prostatic cancer patients. These results show that it is possible to detect 
      numerical chromosomal changes and oncogene amplification in bone marrow 
      micrometastatic epithelial cells of cancer patients by combining 
      immunophenotyping and FISH.
FAU - Muller, P
AU  - Muller P
AD  - II. Medizinische Klinik, Zentralklinikum Augsburg, Germany.
FAU - Weckermann, D
AU  - Weckermann D
FAU - Riethmuller, G
AU  - Riethmuller G
FAU - Schlimok, G
AU  - Schlimok G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Bone Marrow Neoplasms/*genetics/pathology/*secondary
MH  - Breast Neoplasms/genetics
MH  - *Chromosome Aberrations
MH  - Epithelium/pathology
MH  - Female
MH  - Gene Amplification
MH  - Humans
MH  - Immunophenotyping
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Prostatic Neoplasms/genetics
MH  - Receptor, ErbB-2/genetics
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
AID - 0165460895001891 [pii]
AID - 10.1016/0165-4608(95)00189-1 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1996 May;88(1):8-16. doi: 10.1016/0165-4608(95)00189-1.