PMID- 8631159
OWN - NLM
STAT- MEDLINE
DCOM- 19960702
LR  - 20220318
IS  - 0192-253X (Print)
IS  - 0192-253X (Linking)
VI  - 18
IP  - 3
DP  - 1996
TI  - Co-expression of the HGF/SF and c-met genes during early mouse embryogenesis 
      precedes reciprocal expression in adjacent tissues during organogenesis.
PG  - 254-66
AB  - Early experiments with cells in culture and recent targeting experiments have 
      confirmed that the mesenchyme-derived growth factor hepatocyte growth 
      factor/scatter factor (HGF/SF) is a paracrine agent that regulates the 
      development of several epithelial and myogenic precursor cells during 
      organogenesis. Here, we report the expression pattern of HGF/SF and its receptor, 
      the product of the proto-oncogene c-met, during gastrulation and early 
      organogenesis in mouse embryo. During gastrulation, the expression of HGF/SF and 
      c-met overlaps. Initially the two genes are expressed in the endoderm and in the 
      mesoderm along the rostro-intermediate part of the primitive streak and, later, 
      in the node and in the notochord. Neither HGF/SF nor c-met is expressed in the 
      ectodermal layer throughout gastrulation. During early organogenesis, overlapping 
      expression of HGF/SF and c-met is found in heart, condensing somites and neural 
      crest cells. However, a second and distinct pattern of expression, characterized 
      by the presence of the ligand in mesenchymal tissues and the receptor in the 
      surrounding ectoderm, is seen in the bronchial arches and in the limb buds. At 13 
      days postcoitum (d.p.c.), only this second pattern of expression is observed in 
      differentiated somites and several major organs (i.e., lungs, liver, and gut). 
      The expression of the HGF/SF and c-met genes throughout embryogenesis suggests a 
      shift from an autocrine to a paracrine signaling system. The shift takes place in 
      early organogenesis and implies different roles of HGF/SF in development. During 
      gastrulation, HGF/SF may affect the fate of migrating mesodermal cells and may 
      play a role in axis determination, whereas during organogenesis, the expression 
      patterns of HGF/SF and its receptor reflect the recently established roles in the 
      growth of certain epithelia and the migration of specific myogenic precursor 
      cells.
FAU - Andermarcher, E
AU  - Andermarcher E
AD  - ICRF Cell Interactions Laboratory, Cambridge University Medical School, MRC 
      Centre, UK.
FAU - Surani, M A
AU  - Surani MA
FAU - Gherardi, E
AU  - Gherardi E
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Dev Genet
JT  - Developmental genetics
JID - 7909963
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Animals
MH  - Branchial Region/embryology/metabolism
MH  - Cardiovascular System/embryology/metabolism
MH  - Embryonic and Fetal Development/genetics/*physiology
MH  - Female
MH  - Gastrula/metabolism
MH  - *Gene Expression
MH  - Hepatocyte Growth Factor/*genetics
MH  - Limb Buds/embryology/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred CBA
MH  - Proto-Oncogene Proteins c-met
MH  - Receptor Protein-Tyrosine Kinases/*genetics
MH  - Viscera/embryology/metabolism
EDAT- 1996/01/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1002/(SICI)1520-6408(1996)18:3<254::AID-DVG6>3.0.CO;2-8 [pii]
AID - 10.1002/(SICI)1520-6408(1996)18:3<254::AID-DVG6>3.0.CO;2-8 [doi]
PST - ppublish
SO  - Dev Genet. 1996;18(3):254-66. doi: 
      10.1002/(SICI)1520-6408(1996)18:3<254::AID-DVG6>3.0.CO;2-8.