PMID- 8631506
OWN - NLM
STAT- MEDLINE
DCOM- 19960703
LR  - 20071114
IS  - 0012-1606 (Print)
IS  - 0012-1606 (Linking)
VI  - 174
IP  - 2
DP  - 1996 Mar 15
TI  - Multiple actions of stem cell factor in neural crest cell differentiation in 
      vitro.
PG  - 345-59
AB  - The neural crest is a transient tissue of the vertebrate embryo that gives rise 
      to most primary sensory neurons and pigment cells in the adult organism, among 
      other cell types and tissues. Many neural crest cells are pluripotent in the 
      sense that their progeny can generate more than one phenotype. The presence of 
      pluripotent neural crest cell-derived cells at sites of terminal differentiation 
      suggests that location-specific cues from the embryonic environment, such as 
      growth factors, are involved in directing their survival, proliferation, and cell 
      type specification. We have therefore examined the influences of one pertinent 
      growth factor, stem cell factor (SCF), on neural crest cell development by in 
      vitro colony assay in a serum-free culture medium. SCF showed three major 
      effects. (1) SCF is trophic for early neural crest cells, that is, either 
      pluripotent cells and/or their more mature progeny. This effect occurs only if 
      SCF is present throughout the culture period, and it is not observed when a 
      neurotrophin is present in addition to SCF. (2) More colonies contain sensory 
      neuron precursors in the presence of SCF. This effect is neutralized by NGF and 
      neurotrophin-3 (NT-3), but not by brain-derived neurotrophic factor (BDNF). (3) 
      The combination of SCF and any neurotrophin tested (NGF, BDNF, NT-3) is trophic 
      for melanogenic cells, whereas SCF alone does not detectably affect 
      melanogenesis. This suggests either that both types of factor are required for 
      melanotrophic action or that melanogenic cells become dependent on neurotrophins 
      after exposure to SCF. Our observation that SCF is required during the first half 
      of the culture period only, and NGF during the second half only, indicates the 
      latter possibility. Whereas coat color changes in the mouse mutants W (c-kit 
      defect) and Steel (SCF defect) and several in vivo and in vitro studies by other 
      investigators have shown previously that SCF is melanotrophic, they also 
      indicated the requirement of an additional factor, or factors, in melanogenesis. 
      Our data suggest that SCF affects neural crest cell development at multiple 
      levels and that survival of melanogenic cells is mediated by a combination of SCF 
      and a neurotropin, rather than by SCF alone.
FAU - Langtimm-Sedlak, C J
AU  - Langtimm-Sedlak CJ
AD  - Department of Cellular Biology and Anatomy, Medical College of Wisconsin, 
      Milwaukee 53226, USA.
FAU - Schroeder, B
AU  - Schroeder B
FAU - Saskowski, J L
AU  - Saskowski JL
FAU - Carnahan, J F
AU  - Carnahan JF
FAU - Sieber-Blum, M
AU  - Sieber-Blum M
LA  - eng
GR  - N01-HD-6-2915/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Stem Cell Factor)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/*drug effects
MH  - Mice
MH  - Nerve Growth Factors/pharmacology
MH  - Neural Crest/cytology/*drug effects
MH  - Neurotrophin 3
MH  - Quail/embryology
MH  - Stem Cell Factor/*pharmacology
EDAT- 1996/03/15 00:00
MHDA- 1996/03/15 00:01
CRDT- 1996/03/15 00:00
PHST- 1996/03/15 00:00 [pubmed]
PHST- 1996/03/15 00:01 [medline]
PHST- 1996/03/15 00:00 [entrez]
AID - S0012-1606(96)90079-2 [pii]
AID - 10.1006/dbio.1996.0079 [doi]
PST - ppublish
SO  - Dev Biol. 1996 Mar 15;174(2):345-59. doi: 10.1006/dbio.1996.0079.