PMID- 8632056 OWN - NLM STAT- MEDLINE DCOM- 19960702 LR - 20190825 IS - 0165-5728 (Print) IS - 0165-5728 (Linking) VI - 64 IP - 2 DP - 1996 Feb TI - Expression of high- and low-affinity neurotrophin receptors on human transformed B lymphocytes. PG - 141-9 AB - We performed high-sensitivity flow cytometry and Western blotting to study the expression of the low-affinity NGF receptor (p75NGFR) and of the transmembrane tyrosine kinase (Trk) family of high-affinity receptors for the different neurotrophic factors on Epstein-Barr virus (EBV)-transformed human B lymphocytes. Reverse transcriptase (RT) polymerase chain reaction (PCR) with single and multiple sets of primers (multiplex RT-PCR) was used to survey the repertoire of neurotrophin receptor transcripts in this cell line. We demonstrated that transformed B cells express detectable levels of Trk b and its mRNA. Conversely, negative results were obtained for p75NGFR, Trk a, and Trk c. Exposure of EBV-transformed B lymphocytes to brain-derived neurotrophic factor (BDNF) triggered the phosphorylation of Trk b, as demonstrated by Western blots of cell lysates probed with monoclonal antibody against phosphotyrosine. FAU - Schenone, A AU - Schenone A AD - Department of Neurology, Mayo Clinic and Mayo Foundation, Rochester MN 55905, USA. FAU - Gill, J S AU - Gill JS FAU - Zacharias, D A AU - Zacharias DA FAU - Windebank, A J AU - Windebank AJ LA - eng GR - NS 29769/NS/NINDS NIH HHS/United States PT - Journal Article PT - Research Support, U.S. Gov't, P.H.S. PL - Netherlands TA - J Neuroimmunol JT - Journal of neuroimmunology JID - 8109498 RN - 0 (Membrane Glycoproteins) RN - 0 (Membrane Proteins) RN - 0 (Molecular Probes) RN - 0 (Proto-Oncogene Proteins) RN - 0 (RNA, Messenger) RN - 0 (Receptor, Ciliary Neurotrophic Factor) RN - 0 (Receptor, Nerve Growth Factor) RN - 0 (Receptors, Nerve Growth Factor) RN - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases) RN - EC 2.7.10.1 (Receptor, trkA) SB - IM MH - B-Lymphocytes/*metabolism MH - Base Sequence MH - Binding, Competitive MH - Blotting, Western MH - Cell Line, Transformed MH - Cell Transformation, Viral MH - Flow Cytometry MH - Herpesvirus 4, Human MH - Humans MH - Membrane Glycoproteins/metabolism MH - Membrane Proteins/genetics/metabolism MH - Molecular Probes/genetics MH - Molecular Sequence Data MH - Phosphorylation MH - Polymerase Chain Reaction MH - Proto-Oncogene Proteins/metabolism MH - RNA, Messenger/metabolism MH - Receptor Protein-Tyrosine Kinases/metabolism MH - Receptor, Ciliary Neurotrophic Factor MH - Receptor, Nerve Growth Factor MH - Receptor, trkA MH - Receptors, Nerve Growth Factor/*metabolism MH - Transcription, Genetic EDAT- 1996/02/01 00:00 MHDA- 2000/06/01 09:00 CRDT- 1996/02/01 00:00 PHST- 1996/02/01 00:00 [pubmed] PHST- 2000/06/01 09:00 [medline] PHST- 1996/02/01 00:00 [entrez] AID - 016557289500162X [pii] AID - 10.1016/0165-5728(95)00162-x [doi] PST - ppublish SO - J Neuroimmunol. 1996 Feb;64(2):141-9. doi: 10.1016/0165-5728(95)00162-x.