PMID- 8634025
OWN - NLM
STAT- MEDLINE
DCOM- 19960628
LR  - 20220408
IS  - 0304-8608 (Print)
IS  - 0304-8608 (Linking)
VI  - 141
IP  - 2
DP  - 1996
TI  - Human T-cell lymphotropic virus types I and II infections in patients with 
      leukaemia/lymphoma and in subjects with sexually transmitted diseases in Nigeria.
PG  - 345-55
AB  - Serological assays that distinguish antibodies to human T-cell lymphotropic virus 
      types I (HTLV-I) and type II (HTLV-II), and polymerase chain reaction (PCR) tests 
      were used to investigate association of these two human retroviruses with several 
      well-defined clinical conditions in Nigeria. We compared the frequency of HTLV-I 
      and HTLV-II infections among patients with lymphopholiferative disorders (n=65), 
      individuals with various sexually transmitted diseases (n=40), patients with 
      genitals candidiasis (n=25) and apparently healthy individuals (n=60). 
      Serological analysis of blood samples from all four groups showed that 10 of the 
      190 (5.3%) individuals tested were confirmed positive for the presence of 
      antibodies to HTLV-I(6) or HTLV-II(4). Using the PCR technique, specific HTLV-I 
      or HTLV-II sequences were amplified from the genomic DNA of 4 of 6 HTLV-I 
      seropositive and 3 of the 4 HTLV-II seropositive individuals respectively. 
      However, sequences of both viruses were amplified from the genomic DNAs of the 
      remaining 3 seropositive individuals. Since one of the 5 sets of primer pairs 
      [SK110(II)/SK111(II)], which is used for specific identification of HTLV-II did 
      not amplify the target sequence from the genomic DNAs of any of the 4 HTLV-II 
      confirmed seropositive individuals in this study, it suggested sequence diversity 
      of these viruses in Nigeria. The virus-infected individuals identified in this 
      study were one (1.5%) of the 65 patients with leukaemia/lymphoma (HTLV-I), 6 of 
      40 (15.0%) individuals (HTLV-I = 1 , HTLV-II = 3, HTLV-I/II = 2) with sexually 
      transmitted diseases (STD), one of 25(4.0%) subjects with genital candidiasis for 
      HTLV-I and 2 of 60 (33.3%) healthy individuals (one for HTLV-I and one for 
      HTLV-I/II). There was a significant difference (P < 0.025) between the prevalence 
      of HTLV-I/II infections among patients with lymphoma/leukaemia and those who 
      attended STD clinic in Ibadan, Nigeria. This study also suggests that while 
      HTLV-I and HTLV-II may be important sexually transmitted viruses, they may not be 
      specific aetiological agents of the common lymphoproliferative disorders in 
      Nigeria.
FAU - Olaleye, O D
AU  - Olaleye OD
AD  - Department of Virology, College of Medicine, University College Hospital, Ibadan, 
      Nigeria.
FAU - Ekweozor, C C
AU  - Ekweozor CC
FAU - Li, Z L
AU  - Li ZL
FAU - Opala, I E
AU  - Opala IE
FAU - Sheng, Z
AU  - Sheng Z
FAU - Onyemenem, T N
AU  - Onyemenem TN
FAU - Rasheed, S
AU  - Rasheed S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
RN  - 0 (HTLV-I Antibodies)
RN  - 0 (HTLV-II Antibodies)
SB  - IM
MH  - Adult
MH  - Base Sequence
MH  - DNA Primers
MH  - DNA, Viral/blood/isolation & purification
MH  - Female
MH  - HTLV-I Antibodies/blood
MH  - HTLV-I Infections/*complications/diagnosis/epidemiology
MH  - HTLV-II Antibodies/blood
MH  - HTLV-II Infections/*complications/diagnosis/epidemiology
MH  - Human T-lymphotropic virus 1/isolation & purification
MH  - Human T-lymphotropic virus 2/isolation & purification
MH  - Humans
MH  - Leukemia/*complications
MH  - Lymphoma/*complications
MH  - Male
MH  - Molecular Sequence Data
MH  - Nigeria/epidemiology
MH  - Polymerase Chain Reaction
MH  - Prevalence
MH  - Sexually Transmitted Diseases/blood/immunology/*virology
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1007/BF01718404 [doi]
PST - ppublish
SO  - Arch Virol. 1996;141(2):345-55. doi: 10.1007/BF01718404.