PMID- 8635431
OWN - NLM
STAT- MEDLINE
DCOM- 19960709
LR  - 20190813
IS  - 0013-9580 (Print)
IS  - 0013-9580 (Linking)
VI  - 37
IP  - 2
DP  - 1996 Feb
TI  - Change in neurotrophins and their receptor mRNAs in the rat forebrain after 
      status epilepticus induced by pilocarpine.
PG  - 198-207
AB  - We studied the effects of status epilepticus (SE) induced by lithium 
      chloride/pilocarpine treatment on gene expression of neurotrophins of the nerve 
      growth factor (NGF) family and of their high-affinity receptors of the tyrosine 
      protein kinase (trk) family in the forebrain. Using in situ hybridization (ISH), 
      we demonstrated an early (3 h after treatment) increase in brain-derived 
      neurotrophic factor (BDNF) and trkB mRNA expression in the dentate gyrus, 
      amygdala, and piriform cortex, as well as widespread increases in the cerebral 
      cortex. NGF mRNA, but not the mRNA of its receptor trkA, was increased in the 
      dentate gyrus. In contrast, 12 h after treatment, neurotrophin-3 (NT-3) 
      decreased, and its receptor trkC mRNA increased. There was no change in NT-4 mRNA 
      levels. All changes were blocked by pretreatment with scopolamine, a muscarinic 
      antagonist. The noncompetitive N-methyl-D-aspartate (NMDA) antagonist ketamine 
      blocked NGF, BDNF, and trkB mRNA increases in the hippocampus and cerebral 
      cortex, but not in the amygdala and piriform cortex. In contrast, ketamine did 
      not affect NT-3 and trkC changes. These results provide a complete description of 
      changes in mRNA levels of neurotrophins and their receptors in the forebrain 
      after SE and supply additional data supporting the view that neurotrophin gene 
      expression is related to abnormal neuronal activity.
FAU - Mudo, G
AU  - Mudo G
AD  - Institute of Human Physiology, University of Catania, Italy.
FAU - Jiang, X H
AU  - Jiang XH
FAU - Timmusk, T
AU  - Timmusk T
FAU - Bindoni, M
AU  - Bindoni M
FAU - Belluardo, N
AU  - Belluardo N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Epilepsia
JT  - Epilepsia
JID - 2983306R
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurotrophin 3)
RN  - 0 (RNA, Messenger)
RN  - 01MI4Q9DI3 (Pilocarpine)
RN  - 690G0D6V8H (Ketamine)
RN  - DL48G20X8X (Scopolamine)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - G4962QA067 (Lithium Chloride)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor
MH  - Cerebral Cortex/chemistry/drug effects
MH  - Gene Expression
MH  - Hippocampus/chemistry/drug effects
MH  - In Situ Hybridization
MH  - Ketamine/pharmacology
MH  - Lithium Chloride
MH  - Male
MH  - Nerve Growth Factors/*analysis/genetics
MH  - Nerve Tissue Proteins/*analysis/genetics
MH  - Neurotrophin 3
MH  - *Pilocarpine
MH  - Prosencephalon/*chemistry/metabolism
MH  - Protein-Tyrosine Kinases/genetics/metabolism
MH  - RNA, Messenger/*analysis
MH  - Rats
MH  - Rats, Wistar
MH  - Scopolamine/pharmacology
MH  - Status Epilepticus/*chemically induced/genetics/metabolism
MH  - Tissue Distribution
EDAT- 1996/02/01 00:00
MHDA- 1996/02/01 00:01
CRDT- 1996/02/01 00:00
PHST- 1996/02/01 00:00 [pubmed]
PHST- 1996/02/01 00:01 [medline]
PHST- 1996/02/01 00:00 [entrez]
AID - 10.1111/j.1528-1157.1996.tb00012.x [doi]
PST - ppublish
SO  - Epilepsia. 1996 Feb;37(2):198-207. doi: 10.1111/j.1528-1157.1996.tb00012.x.