PMID- 8638662
OWN - NLM
STAT- MEDLINE
DCOM- 19960705
LR  - 20191210
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 270
IP  - 3 Pt 1
DP  - 1996 Mar
TI  - Modulation of cell membrane potential in cultured vascular endothelium.
PG  - C819-24
AB  - The present study explores the role of different ionic conductances in the 
      regulation of membrane potential under resting conditions and after bradykinin 
      (BK) or thapsigargin (TG) stimulation of cultured bovine aortic endothelial 
      cells. Under resting conditions, the cell membrane potential observed was -62+/- 
      5 mV. The main conductance under these conditions is an inwardly rectifying 
      potassium (IRK) channel. Application of 50 nM BK induced a transient 
      hyperpolarization to -87 +/- 4 mV followed by sustained depolarization to -35 +/- 
      5 mV. The transient hyperpolarization was eliminated by 1 microM noxiustoxin, a 
      blocker of calcium-activated postassium channels (K(Ca)). the sustained 
      depolarization induced by BK was prevented by incubating the cells with the 
      calcium channel blocker lanthanum. TG evoked a similar response in membrane 
      potential, with the exception that the onset of the hyperpolarization was slower 
      compared with BK. The results presented here indicate that the cell resting 
      potential is maintained at -62 +/- 2 mV by the IRK channel. BK or TG stimulation 
      induces a transient hyperpolarization of approximately -20 mV produced by 
      activation of a KCa. This hyperpolarization is followed by a sustained 
      depolarization produced by activation of a calcium-selective channel sensitive to 
      lanthanum.
FAU - Vaca, L
AU  - Vaca L
AD  - Department of Molecular Physiology and Biophysics, Baylor College of Medicine, 
      Houston, Texas 77030, USA.
FAU - Licea, A
AU  - Licea A
FAU - Possani, L D
AU  - Possani LD
LA  - eng
GR  - HL-51949/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Potassium Channel Blockers)
RN  - 0 (Potassium Channels)
RN  - 0 (Potassium Channels, Inwardly Rectifying)
RN  - 0 (Scorpion Venoms)
RN  - 0 (Terpenes)
RN  - 526U7A2651 (Egtazic Acid)
RN  - 67526-95-8 (Thapsigargin)
RN  - 6I3K30563S (Lanthanum)
RN  - 85205-49-8 (noxiustoxin)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - S8TIM42R2W (Bradykinin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Aorta
MH  - Bradykinin/pharmacology
MH  - Calcium/pharmacology
MH  - Calcium-Transporting ATPases/antagonists & inhibitors
MH  - Cattle
MH  - Cell Membrane/*physiology
MH  - Cells, Cultured
MH  - Egtazic Acid/pharmacology
MH  - Electric Conductivity
MH  - Endothelium, Vascular/drug effects/*physiology
MH  - Enzyme Inhibitors/pharmacology
MH  - Kinetics
MH  - Lanthanum/pharmacology
MH  - Membrane Potentials
MH  - Potassium Channel Blockers
MH  - Potassium Channels/*physiology
MH  - *Potassium Channels, Inwardly Rectifying
MH  - Scorpion Venoms/pharmacology
MH  - Terpenes/pharmacology
MH  - Thapsigargin
MH  - Time Factors
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
AID - 10.1152/ajpcell.1996.270.3.C819 [doi]
PST - ppublish
SO  - Am J Physiol. 1996 Mar;270(3 Pt 1):C819-24. doi: 10.1152/ajpcell.1996.270.3.C819.