PMID- 8639172
OWN - NLM
STAT- MEDLINE
DCOM- 19960708
LR  - 20190718
IS  - 0004-3591 (Print)
IS  - 0004-3591 (Linking)
VI  - 39
IP  - 5
DP  - 1996 May
TI  - Anti-endothelial cell IgG antibodies from patients with Wegener's granulomatosis 
      bind to human endothelial cells in vitro and induce adhesion molecule expression 
      and cytokine secretion.
PG  - 758-66
AB  - OBJECTIVE: To elucidate the role of anti-endothelial cell antibodies (AECA) in 
      vascular inflammation in patients with Wegener's granulomatosis (WG). METHODS: 
      IgG fractions from 3 AECA-positive WG patients, IgG from 3 AECA-negative WG 
      patients, and IgG from healthy donors were tested for their ability to: a) bind 
      to endothelial cells and to display complement-dependent or antibody-dependent 
      cellular cytotoxicity, b) modulate cell membrane expression of adhesion 
      molecules, as evaluated by cytofluorometry and by immunoenzymatic assay, and c) 
      induce the secretion of interleukin-1 beta (IL-1 beta), IL-6, IL-8, and monocyte 
      chemotactic protein 1 (MCP-1). RESULTS: We found that AECA IgG from WG patients 
      do not display any significant cytotoxicity but are able to up-regulate the 
      expression of E-selectin, intercellular adhesion molecule 1 and vascular cell 
      adhesion molecule 1 and to induce the secretion of IL-1 beta, IL-6, IL-8, and 
      MCP-1. CONCLUSION: AECA in patients with WG could play a potential pathogenetic 
      role by activating endothelial cells, and thus facilitating leukocyte recruitment 
      and adhesion to endothelial surfaces, rather than by displaying a cytotoxic 
      activity.
FAU - Del Papa, N
AU  - Del Papa N
AD  - Instituto di Medicina Interna, University of Milan, Italy.
FAU - Guidali, L
AU  - Guidali L
FAU - Sironi, M
AU  - Sironi M
FAU - Shoenfeld, Y
AU  - Shoenfeld Y
FAU - Mantovani, A
AU  - Mantovani A
FAU - Tincani, A
AU  - Tincani A
FAU - Balestrieri, G
AU  - Balestrieri G
FAU - Radice, A
AU  - Radice A
FAU - Sinico, R A
AU  - Sinico RA
FAU - Meroni, P L
AU  - Meroni PL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arthritis Rheum
JT  - Arthritis and rheumatism
JID - 0370605
RN  - 0 (Autoantibodies)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Cytokines)
RN  - 0 (Immunoglobulin G)
RN  - 0 (anti-endothelial cell antibody)
SB  - IM
MH  - Autoantibodies/*analysis/physiology
MH  - Cell Adhesion Molecules/*metabolism
MH  - Cells, Cultured
MH  - Cytokines/*metabolism
MH  - Cytotoxicity, Immunologic
MH  - Endothelium, Vascular/chemistry/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Granulomatosis with Polyangiitis/blood/*immunology/metabolism
MH  - Humans
MH  - Immunoglobulin G/immunology
MH  - Umbilical Veins/chemistry/metabolism
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
AID - 10.1002/art.1780390507 [doi]
PST - ppublish
SO  - Arthritis Rheum. 1996 May;39(5):758-66. doi: 10.1002/art.1780390507.