PMID- 8639450
OWN - NLM
STAT- MEDLINE
DCOM- 19960715
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 93
IP  - 2
DP  - 1996 May
TI  - Association of autoantibodies against platelet glycoproteins Ib/IX and IIb/IIIa, 
      and platelet-reactive anti-HIV antibodies in thrombocytopenic narcotic addicts.
PG  - 464-71
AB  - The levels of platelet-associated Igs (PAIgs) and plasma circulating antiplatelet 
      antibodies were evaluated by a flow cytometric immunofluorescence assay (FCIFA), 
      an enzyme-linked immunoassay (ELISA), and a platelet radioactive antiglobulin 
      test (PRAT), in a group of 45 human immunodeficiency virus (HIV)-infected 
      intravenous drug users (IVDUs), with or without thrombocytopenia (TCP). Direct 
      tests demonstrated an increased amount of PAIgs in 40% of the patients, 
      irrespective of their platelet count. These PAIgs were mainly of IgG class and 
      could not be eluted with ether. Plasma IgG with antiplatelet activity was found 
      in 70% of the thrombocytopenic individuals, whereas it was detected in only one 
      patient without TCP. The relative frequencies of antibodies against the platelet 
      glycoproteins (GPs) Ib/IX and IIb/IIIa were assessed in plasma from all patients 
      by means of the monoclonal antibody-specific immobilization of platelet antigens 
      assay (MAIPA). Plasmas from all non-thrombocytopenic patients were negative when 
      tested by indirect MAIPA. In contrast, 10/23 plasma from thrombocytopenic 
      patients reacted with either GP IIb/IIIa, GP Ib/IX, or both GPs. Finally, aiming 
      to investigate whether HIV antibodies from these patients are reactive with 
      normal platelets, we performed absorption-elution experiments, followed by 
      evaluation of HIV antibodies in the indirect eluates by ELISA and Western blot. 
      Interestingly, we detected anti-HIV antibodies that bind to normal platelet 
      antigens in 50% of the ether eluates prepared from control platelets sensitized 
      with plasma from patients with TCP, but in only 5% of eluates obtained from 
      platelets sensitized with plasma from non-thrombocytopenic patients. The present 
      study provides direct evidence that specific autoantibodies against platelet 
      membrane GPs Ib/IX and IIb/IIIa are common in HIV positive thrombocytopenic 
      individuals. The finding in these patients of HIV antibodies cross-reactive with 
      normal platelets, suggests that mimicry of human antigens by HIV could play a key 
      role in the pathophysiology of the HIV-related TCP.
FAU - Gonzalez-Conejero, R
AU  - Gonzalez-Conejero R
AD  - Unit of Haematology and Haemotherapy, Hospital General Universitario, Murcia, 
      Spain.
FAU - Rivera, J
AU  - Rivera J
FAU - Rosillo, M C
AU  - Rosillo MC
FAU - Cano, A
AU  - Cano A
FAU - Rodriguez, T
AU  - Rodriguez T
FAU - Vicente, V
AU  - Vicente V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Antigens, Human Platelet)
RN  - 0 (Autoantibodies)
RN  - 0 (HIV Antibodies)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Platelet Glycoprotein GPIIb-IIIa Complex)
RN  - 0 (Platelet Glycoprotein GPIb-IX Complex)
SB  - IM
MH  - Adult
MH  - Antigens, Human Platelet
MH  - Autoantibodies/*analysis/immunology
MH  - Blotting, Western
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - HIV Antibodies/*analysis/immunology
MH  - Humans
MH  - Immunoglobulin G/analysis
MH  - Male
MH  - Opioid-Related Disorders/complications/*immunology
MH  - Platelet Glycoprotein GPIIb-IIIa Complex/*immunology
MH  - Platelet Glycoprotein GPIb-IX Complex/*immunology
MH  - Substance Abuse, Intravenous/complications/immunology
MH  - Thrombocytopenia/*immunology/virology
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
AID - 10.1046/j.1365-2141.1996.00502.x [doi]
PST - ppublish
SO  - Br J Haematol. 1996 May;93(2):464-71. doi: 10.1046/j.1365-2141.1996.00502.x.