PMID- 8639485
OWN - NLM
STAT- MEDLINE
DCOM- 19960715
LR  - 20101118
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 35
IP  - 11
DP  - 1996 Mar 19
TI  - Molecular cloning and functional expression of a new human CC-chemokine receptor 
      gene.
PG  - 3362-7
AB  - The cloning of several receptors activated by either CC or CXC chemokines and 
      belonging to the G protein-coupled family of receptors has been reported 
      recently. In the present work, we describe the cloning of a human gene, named 
      ChemR13, encoding a new CC-chemokine receptor. The gene encodes a protein of 352 
      amino acids with a calculated molecular mass of 40 600 Da and displaying a single 
      potential site for N-linked glycosylation. Using a set of overlapping lambda 
      clones, the genomic organisation of the locus was investigated, demonstrating 
      that the ChemR13 gene is physically linked, and in the same orientation, as the 
      CC-CKR2 gene that encodes a receptor for the monocyte chemoattractant protein-1 
      (MCP-1). A distance of 17.5 kb separates the two coding regions, which share 75% 
      identity in nucleic acid and amino acid sequences. Human ChemR13 was functionally 
      expressed in a stably transfected CHO-K1 cell line. Physiological responses to 
      chemokines were monitored using a microphysiometer. Macrophage inflammatory 
      protein 1 alpha (MIP-1 alpha) was the most potent agonist. MIP-1 beta and RANTES 
      were also active at physiological concentrations. The other CC-chemokines, MCP-1, 
      MCP-2 and MCP-3, as well as CXC-chemokines (IL-8, GRO alpha) had no effect. 
      ChemR13 receptor transcripts were detected by Northern blotting in the 
      promyeloblastic cell line KG-1A, suggesting a potential role in the control of 
      granulocytic lineage proliferation or differentiation. ChemR13 is thus a new 
      member of the growing family of chemokine receptors that mediate the recruitment 
      of cells involved in immune and inflammatory processes. Being the fifth 
      functionally identified receptor in his class, this new CC-chemokine receptor 
      (CC-CKR) is tentatively designated CC-CKR5.
FAU - Samson, M
AU  - Samson M
AD  - IRIBHN, Universite libre de Bruxelles, Beligum.
FAU - Labbe, O
AU  - Labbe O
FAU - Mollereau, C
AU  - Mollereau C
FAU - Vassart, G
AU  - Vassart G
FAU - Parmentier, M
AU  - Parmentier M
LA  - eng
SI  - GENBANK/U66285
SI  - GENBANK/X91492
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, CCR5)
RN  - 0 (Receptors, Cytokine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - CHO Cells
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - DNA Primers/chemistry
MH  - Gene Expression
MH  - Genes
MH  - Genetic Linkage
MH  - Humans
MH  - Molecular Sequence Data
MH  - Multigene Family
MH  - RNA, Messenger/genetics
MH  - Receptors, CCR5
MH  - Receptors, Cytokine/*genetics
MH  - Restriction Mapping
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
EDAT- 1996/03/19 00:00
MHDA- 1996/03/19 00:01
CRDT- 1996/03/19 00:00
PHST- 1996/03/19 00:00 [pubmed]
PHST- 1996/03/19 00:01 [medline]
PHST- 1996/03/19 00:00 [entrez]
AID - bi952950g [pii]
AID - 10.1021/bi952950g [doi]
PST - ppublish
SO  - Biochemistry. 1996 Mar 19;35(11):3362-7. doi: 10.1021/bi952950g.