PMID- 8640717
OWN - NLM
STAT- MEDLINE
DCOM- 19960718
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 88
IP  - 2
DP  - 1996 Jun
TI  - MLL/ENL fusion in congenital acute lymphoblastic leukemia with a unique 
      t(11;18;19).
PG  - 103-9
AB  - To elucidate the events leading to a unique complex translocation involving 
      chromosomes 11, 18, and 19 in a congenital progenitor B-cell acute lymphoblastic 
      leukemia, we have performed comprehensive cytogenetic and fluorescence in situ 
      hybridization (FISH) analyses as well as molecular genetic studies on the DNA and 
      RNA level. We were able to confirm the cytogenetic interpretation of this complex 
      t(11;18;19)(q23;q22;p13.3) by chromosome painting. Involvement of the MLL gene on 
      11q23 became evident by Southern blot analysis as well as by FISH with a YAC 
      clone containing the respective gene. Despite the fact that the additional 
      signals of the split YAC clone were observed on the abnormal chromosome 18, 
      reverse transcription polymerase chain reaction (RT-PCR) revealed a MLL/ENL 
      hybrid mRNA, which is specific for a t(11;19)(q23;p13.3). This gene fusion most 
      probably represents the critical part of this rearrangement. The transfer of the 
      translocated part of the split YAC clone onto chromosome 18 indicates that the 
      second break must have occurred in the vicinity of the first one, at a distance 
      too close to be resolved by FISH. Whether this break took place within chromosome 
      11 or 19 sequences, up- or downstream of the MLL/ENL fusion, and whether this 
      translocation results from a concerted simultaneous exchange of material or from 
      two separate sequential events in consecutive cell generations remains open.
FAU - Horstmann, M
AU  - Horstmann M
AD  - Department of Hematology and Oncology, Children's Hospital, University Hamburg, 
      Germany.
FAU - Argyriou-Tirita, A
AU  - Argyriou-Tirita A
FAU - Borkhardt, A
AU  - Borkhardt A
FAU - Kabisch, H
AU  - Kabisch H
FAU - Kapaun, P
AU  - Kapaun P
FAU - Winkler, K
AU  - Winkler K
FAU - Haas, O A
AU  - Haas OA
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Blotting, Southern
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - Chromosomes, Human, Pair 18/*genetics
MH  - Chromosomes, Human, Pair 19/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant, Newborn
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/congenital/*genetics
MH  - Translocation, Genetic/*genetics
EDAT- 1996/06/01 00:00
MHDA- 1996/06/01 00:01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 1996/06/01 00:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
AID - 0165460895002766 [pii]
AID - 10.1016/0165-4608(95)00276-6 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1996 Jun;88(2):103-9. doi: 10.1016/0165-4608(95)00276-6.