PMID- 8641364
OWN - NLM
STAT- MEDLINE
DCOM- 19960717
LR  - 20071114
IS  - 0301-472X (Print)
IS  - 0301-472X (Linking)
VI  - 24
IP  - 2
DP  - 1996 Feb
TI  - Influence of retroviral-mediated gene transduction of both the recombinant human 
      erythropoietin receptor and interleukin-9 receptor genes into single 
      CD34++CD33-or low cord blood cells on cytokine-stimulated erythroid colony 
      formation.
PG  - 347-51
AB  - Introduction of genes for cytokine receptors into hematopoietic stem/progenitor 
      cells (HSC/HPC) may be of clinical use in the future. We recently reported that 
      retroviral-mediated transduction of either the human erythropoietin receptor 
      (hEpoR) or interleukin-9 receptor (hIL-9R) genes into highly purified HSC/HPC 
      from cord blood (CB) resulted in increased numbers of detectable 
      cytokine-responsive erythroid progenitors (burst-forming units-erythroid 
      [BFU-E]). In the present study, we evaluated if this increase could be further 
      enhanced by cotransducing both these genes into single isolated HSC/HPC. Single 
      CD34++CD33-or low-expressing cells from CB were transduced with viral supernatant 
      containing the hEpoR or hIL-9R genes or cotransduced with both genes. In the 
      presence of Steel factor (SLF), interleukin-3 (IL-3), granulocyte-macrophage 
      colony-stimulating factor (GM-CSF), erythropoietin (Epo), and IL-9, the numbers 
      of erythroid colonies formed were significantly increased after transduction of 
      cells with either the hIL-9R or hEpoR gene compared to mock-transduced cells. 
      This increase was significantly enhanced in cells cotransduced with both genes 
      compared with either gene alone. Integration and expression of both genes was 
      confirmed by polymerase chain reaction (PCR) and reverse-transcriptase (RT)-PCR 
      analysis, respectively. The data demonstrate that myeloid progenitors can be 
      transduced at the single-cell level with both hEpoR and hIL-9R genes with 
      resultant enhanced proliferation of these progenitors in the erythroid lineage by 
      combinations of cytokines including Epo and IL-9.
FAU - Lu, L
AU  - Lu L
AD  - Department of Medicine (Hematology/Oncology), Indiana University School of 
      Medicine, Indianapolis 46202-5121, USA.
FAU - Ge, Y
AU  - Ge Y
FAU - Li, Z H
AU  - Li ZH
FAU - Xiao, M
AU  - Xiao M
FAU - Broxmeyer, H E
AU  - Broxmeyer HE
LA  - eng
GR  - R01 HL-46549/HL/NHLBI NIH HHS/United States
GR  - R01 HL-49202/HL/NHLBI NIH HHS/United States
GR  - R37 CA36464/CA/NCI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Exp Hematol
JT  - Experimental hematology
JID - 0402313
RN  - 0 (Antigens, CD34)
RN  - 0 (Hematopoietic Cell Growth Factors)
RN  - 0 (IL9R protein, human)
RN  - 0 (Interleukin-3)
RN  - 0 (Interleukin-9)
RN  - 0 (Receptors, Erythropoietin)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-9)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Stem Cell Factor)
RN  - 11096-26-7 (Erythropoietin)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Antigens, CD34
MH  - Base Sequence
MH  - Cell Differentiation/drug effects
MH  - Cell Division/drug effects
MH  - Cell Separation
MH  - Clone Cells/drug effects
MH  - Colony-Forming Units Assay
MH  - Erythropoiesis/*drug effects
MH  - Erythropoietin/pharmacology
MH  - Fetal Blood/*cytology
MH  - Genetic Vectors
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Hematopoietic Cell Growth Factors/*pharmacology
MH  - Hematopoietic Stem Cells/classification/*metabolism
MH  - Humans
MH  - Interleukin-3/pharmacology
MH  - Interleukin-9/*pharmacology
MH  - Molecular Sequence Data
MH  - Receptors, Erythropoietin/biosynthesis/*genetics
MH  - Receptors, Interleukin/biosynthesis/*genetics
MH  - Receptors, Interleukin-9
MH  - Recombinant Fusion Proteins/*metabolism
MH  - Retroviridae/genetics
MH  - Stem Cell Factor/pharmacology
MH  - Transfection
EDAT- 1996/02/01 00:00
MHDA- 1996/02/01 00:01
CRDT- 1996/02/01 00:00
PHST- 1996/02/01 00:00 [pubmed]
PHST- 1996/02/01 00:01 [medline]
PHST- 1996/02/01 00:00 [entrez]
PST - ppublish
SO  - Exp Hematol. 1996 Feb;24(2):347-51.