PMID- 8641689
OWN - NLM
STAT- MEDLINE
DCOM- 19960712
LR  - 20190722
IS  - 0340-6717 (Print)
IS  - 0340-6717 (Linking)
VI  - 97
IP  - 6
DP  - 1996 Jun
TI  - Genetic mapping studies of 40 loci and 23 cosmids in chromosome 11p13-11q13, and 
      exclusion of mu-calpain as the multiple endocrine neoplasia type 1 gene.
PG  - 732-41
AB  - Forty loci (16 polymorphic and 24 non-polymorphic) together with 23 cosmids 
      isolated from a chromosome 11-specific library were used to construct a detailed 
      genetic map of 11p13-11q13. The map was constructed by using a panel of 13 
      somatic cell hybrids that sub-divided this region into 19 intervals, a meiotic 
      mapping panel of 33 multiple endocrine neoplasia type 1 (MEN1) families (134 
      affected and 269 unaffected members) and a mitotic mapping panel that was used to 
      identify loss of heterozygosity in 38 MEN1-associated tumours. The results 
      defined the most likely order of the 16 loci as being: 11pter-D11S871-(D11S288, 
      D11S149)-11cen-CNTF-PGA-ROM1-D11S480-PYGM- SEA-D11S913-D11S970-D11S97- 
      D11S146-INT2-D11S971-D11S533-11qter. The meiotic mapping studies indicated that 
      the most likely location of the MEN1 gene was in the interval flanked by PYGM and 
      D11S97, and the results of mitotic mapping suggested a possible location of the 
      MEN1 gene telomeric to SEA. Mapping studies of the gene encoding mu-calpain 
      (CAPN1) located CAPN1 to 11q13 and in the vicinity of the MEN1 locus. However, 
      mutational analysis studies did not detect any germ-line CAPN1 DNA sequence 
      abnormalities in 47 unrelated MEN1 patients and the results therefore exclude 
      CAPN1 as the MEN1 gene. The detailed genetic map that has been constructed of the 
      11p13-11q13 region should facilitate the construction of a physical map and the 
      identification of candidate genes for disease loci mapped to this region.
FAU - Pang, J T
AU  - Pang JT
AD  - MRC Molecular Endocrinology Group, Royal Postgraduate Medical School, Hammersmith 
      Hospital, London, UK.
FAU - Lloyd, S E
AU  - Lloyd SE
FAU - Wooding, C
AU  - Wooding C
FAU - Farren, B
AU  - Farren B
FAU - Pottinger, B
AU  - Pottinger B
FAU - Harding, B
AU  - Harding B
FAU - Leigh, S E
AU  - Leigh SE
FAU - Pook, M A
AU  - Pook MA
FAU - Benham, F J
AU  - Benham FJ
FAU - Gillett, G T
AU  - Gillett GT
FAU - Taggart, R T
AU  - Taggart RT
FAU - Thakker, R V
AU  - Thakker RV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Hum Genet
JT  - Human genetics
JID - 7613873
RN  - EC 3.4.22.- (Calpain)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Calpain/*genetics
MH  - Chromosome Mapping/*methods
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - Cosmids/*genetics
MH  - Female
MH  - Gastrinoma/genetics
MH  - Genetic Linkage
MH  - Germ-Line Mutation/genetics
MH  - Humans
MH  - Hybrid Cells
MH  - Insulinoma/genetics
MH  - Male
MH  - Meiosis
MH  - Mitosis
MH  - Molecular Sequence Data
MH  - Multiple Endocrine Neoplasia Type 1/*genetics
MH  - Pancreatic Neoplasms/genetics
MH  - Parathyroid Neoplasms/genetics
MH  - Pedigree
MH  - Pituitary Neoplasms/genetics
MH  - Polymorphism, Genetic
MH  - Sequence Deletion/genetics
EDAT- 1996/06/01 00:00
MHDA- 1996/06/01 00:01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 1996/06/01 00:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
AID - 10.1007/BF02346182 [doi]
PST - ppublish
SO  - Hum Genet. 1996 Jun;97(6):732-41. doi: 10.1007/BF02346182.