PMID- 8642785
OWN - NLM
STAT- MEDLINE
DCOM- 19960716
LR  - 20220321
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 74
IP  - 5
DP  - 1996 May
TI  - Osteogenic potential of murine osteosarcoma cells: comparison of bone-specific 
      gene expression in in vitro and in vivo conditions.
PG  - 895-906
AB  - Bone tissue formation and the expression of osteoblast-specific genes were 
      compared in vitro and in vivo for two well characterized murine clonal 
      osteosarcoma cell lines (K7 and K8). In vitro studies were carried out under 
      conditions that promoted extracellular matrix morphogenesis and mineralization. 
      The K8 cells showed 8-fold greater alkaline phosphatase activity and mineral 
      accumulation than did K7 cells during 21 days of in vitro growth. The K8 cell 
      line showed high levels of bone sialoprotein (BSP), collagen type I (COL I), and 
      alkaline phosphatase (APase) mRNA expression throughout its growth in vitro. In 
      contrast, K7 cells showed an almost complete absence of BSP and COL I and very 
      low levels of APase throughout the culture period. In vitro, both cell lines 
      expressed very low levels of osteocalcin (OC). For in vivo studies, we used a 
      three-dimensional culture device that permitted analysis of tissue formation by 
      the cells after their implantation into syngeneic mice. The K8 cells consistently 
      generated extensive mineralized woven bone after their subcutaneous implantation. 
      The striking features distinguishing the bone formed by the implanted cells from 
      normal recipient bone were the complete absence of osteoclasts or matrix 
      resorption, the absence of OC protein, and very low levels of OC mRNA expression 
      in the tissues formed by these cell lines. BSP, APase, and COL I expressions were 
      maintained at high levels in the K8-produced tissues. In contrast to their near 
      absence in vitro, APase, BSP, and COL I were expressed by K7 cells and increased 
      with time in vivo. These findings demonstrate that the K7 cells in vitro are less 
      differentiated than K8 cells, but that K7 cells in vivo undergo osteogenic 
      maturation. Thus, expression of bone-specific genes in these osteogenic cell 
      lines was dependent on systemic or local factors in recipient animals and was 
      distinct for these cell lines when grown under in vitro conditions. OC protein 
      does not appear to be needed for the mineralization of the extracellular matrix 
      but may be needed to provide the necessary signals for the resorption and 
      remodeling of the tissue.
FAU - Gerstenfeld, L C
AU  - Gerstenfeld LC
AD  - Laboratory for the Study of Skeletal Disorders and Rehabilitation, Children's 
      Hospital, Boston, Massachusetts 02115, USA.
FAU - Uporova, T
AU  - Uporova T
FAU - Schmidt, J
AU  - Schmidt J
FAU - Strauss, P G
AU  - Strauss PG
FAU - Shih, S D
AU  - Shih SD
FAU - Huang, L F
AU  - Huang LF
FAU - Gundberg, C
AU  - Gundberg C
FAU - Mizuno, S
AU  - Mizuno S
FAU - Glowacki, J
AU  - Glowacki J
LA  - eng
GR  - P01 AR 34078/AR/NIAMS NIH HHS/United States
GR  - R01 AR 43434/AR/NIAMS NIH HHS/United States
GR  - R01 DE 08798/DE/NIDCR NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (Sialoglycoproteins)
RN  - 0 (Spp1 protein, mouse)
RN  - 104982-03-8 (Osteocalcin)
RN  - 106441-73-0 (Osteopontin)
RN  - 63231-63-0 (RNA)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Alkaline Phosphatase/genetics
MH  - Animals
MH  - Bone Matrix/metabolism
MH  - Bone Neoplasms/genetics/*metabolism/pathology
MH  - Calcification, Physiologic
MH  - Calcium/analysis
MH  - Collagen/genetics
MH  - Electrophoresis, Agar Gel
MH  - *Gene Expression
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neoplasm Transplantation
MH  - Osteoblasts/*cytology/metabolism
MH  - Osteocalcin/analysis/genetics
MH  - *Osteogenesis
MH  - Osteopontin
MH  - Osteosarcoma/genetics/*metabolism/pathology
MH  - RNA/analysis/genetics
MH  - Sialoglycoproteins/genetics
MH  - Tumor Cells, Cultured
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
PST - ppublish
SO  - Lab Invest. 1996 May;74(5):895-906.