PMID- 8643683
OWN - NLM
STAT- MEDLINE
DCOM- 19960717
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 93
IP  - 4
DP  - 1996 Feb 20
TI  - Immunocytochemical localization of the endogenous neuroexcitotoxin quinolinate in 
      human peripheral blood monocytes/macrophages and the effect of human T-cell 
      lymphotropic virus type I infection.
PG  - 1636-41
AB  - Quinolinate (Quin), a metabolite in the kynurenine pathway of tryptophan 
      degradation and a neurotoxin that appears to act through the N-methyl-D-aspartate 
      receptor system, was localized in cultured human peripheral blood 
      monocytes/macrophages (PBMOs) by using a recently developed immunocytochemical 
      method. Quin immunoreactivity (Quin-IR) was increased in gamma interferon 
      (IFN-gamma)-stimulated monocytes/macrophages (MOs). In addition, the precursors, 
      tryptophan and kynurenine, significantly increased Quin-IR. Infection of MOs by 
      human T-cell lymphotropic virus type I (HTLV-I) in vitro substantially increased 
      both the number of Quin-IR cells and the intensity of Quin-IR. At the peak of the 
      Quin-IR response, about 40% of the cells were Quin-IR positive. In contrast, only 
      about 2-5% of the cells were positive for HTLV-I, as detected by both 
      immunofluorescence for the HTLV-I antigens and PCR techniques for the HTLV-I Tax 
      gene. These results suggest that HTLV-I-induced Quin production in MOs occurs by 
      an indirect mechanism, perhaps via cytokines produced by the infection but not 
      directly by the virus infection per se. The significance of these findings to the 
      neuropathology of HTLV-I infection is discussed.
FAU - Venkateshan, C N
AU  - Venkateshan CN
AD  - Laboratory of Central Nervous System Studies, National Institute of Neurological 
      Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
FAU - Narayanan, R
AU  - Narayanan R
FAU - Espey, M G
AU  - Espey MG
FAU - Moffett, J R
AU  - Moffett JR
FAU - Gajdusek, D C
AU  - Gajdusek DC
FAU - Gibbs, C J Jr
AU  - Gibbs CJ Jr
FAU - Namboodiri, M A
AU  - Namboodiri MA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA, Viral)
RN  - 343-65-7 (Kynurenine)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 8DUH1N11BX (Tryptophan)
RN  - F6F0HK1URN (Quinolinic Acid)
SB  - IM
MH  - Cells, Cultured
MH  - DNA, Viral/analysis
MH  - Human T-lymphotropic virus 1/genetics/*physiology
MH  - Humans
MH  - Interferon-gamma/pharmacology
MH  - Kynurenine/metabolism
MH  - Macrophages/drug effects/*metabolism/ultrastructure/virology
MH  - Monocytes/drug effects/*metabolism/ultrastructure/virology
MH  - Neuroimmunomodulation
MH  - Polymerase Chain Reaction
MH  - Proviruses/genetics/isolation & purification
MH  - Quinolinic Acid/*blood
MH  - Tryptophan/metabolism
PMC - PMC39994
EDAT- 1996/02/20 00:00
MHDA- 2001/03/28 10:01
PMCR- 1996/08/20
CRDT- 1996/02/20 00:00
PHST- 1996/02/20 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1996/02/20 00:00 [entrez]
PHST- 1996/08/20 00:00 [pmc-release]
AID - 10.1073/pnas.93.4.1636 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1996 Feb 20;93(4):1636-41. doi: 10.1073/pnas.93.4.1636.