PMID- 8644112
OWN - NLM
STAT- MEDLINE
DCOM- 19960718
LR  - 20190819
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 108
IP  - 1-2
DP  - 1996 Apr 15
TI  - Further evaluation of the local lymph node assay in the final phase of an 
      international collaborative trial.
PG  - 141-52
AB  - The local lymph node assay (LLNA) is a method used for the prospective 
      identification in mice of chemicals that have the potential to cause skin 
      sensitization. We report here the results of the second and final phase of an 
      international trial in which the performance of the assay has been evaluated 
      using seven test materials in five independent laboratories. The additional 
      chemicals examined here included compounds which are considered less potent 
      allergens than some of those tested in the first phase of the investigation, and 
      includes hexylcinnamic aldehyde (HCA), a chemical recommended by the Organization 
      for Economic Cooperation and Development (OECD) as a positive control for skin 
      sensitization studies. In each laboratory all skin sensitizing chemicals examined 
      (2,4-dinitrochlorobenzene DNCB, HCA, oxazolone, isoeugenal and eugenol) 
      elicited positive responses of comparable magnitude as judged by the derived 
      lowest concentration of test chemical required to elicit a 3-fold or greater 
      increase in the proliferative activity of draining lymph node cells compared with 
      vehicle-treated controls. We observed that sodium lauryl sulphate, considered to 
      be a non-sensitizing skin irritant, also induced a positive response in the 
      assay. Para-aminobenzoic acid (pABA), a nonsensitizing chemical, was negative at 
      all test concentrations in each laboratory. Some laboratories incorporated minor 
      modifications into the standard assay procedure, including the evaluation of 
      lymph nodes pooled from individual mice rather than treatment groups and the use 
      of statistical analyses. The use of statistics did not markedly change the 
      determination of the lowest concentration yielding a positive response. These 
      data confirm that the local lymph node assay is robust and yields equivalent 
      results when performed independently.
FAU - Loveless, S E
AU  - Loveless SE
AD  - E.I. du Pont de Nemours, Inc. Haskell Laboratory, Newark, DE 19711-0050, USA.
FAU - Ladics, G S
AU  - Ladics GS
FAU - Gerberick, G F
AU  - Gerberick GF
FAU - Ryan, C A
AU  - Ryan CA
FAU - Basketter, D A
AU  - Basketter DA
FAU - Scholes, E W
AU  - Scholes EW
FAU - House, R V
AU  - House RV
FAU - Hilton, J
AU  - Hilton J
FAU - Dearman, R J
AU  - Dearman RJ
FAU - Kimber, I
AU  - Kimber I
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (Irritants)
SB  - IM
MH  - Animals
MH  - Data Interpretation, Statistical
MH  - Dermatitis, Allergic Contact/immunology
MH  - Dose-Response Relationship, Drug
MH  - Evaluation Studies as Topic
MH  - Female
MH  - International Cooperation
MH  - Irritants/administration & dosage/*toxicity
MH  - Lymph Nodes/*drug effects/pathology
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Skin Tests/methods
MH  - Toxicity Tests/*methods
MH  - United Kingdom
MH  - United States
EDAT- 1996/04/15 00:00
MHDA- 1996/04/15 00:01
CRDT- 1996/04/15 00:00
PHST- 1996/04/15 00:00 [pubmed]
PHST- 1996/04/15 00:01 [medline]
PHST- 1996/04/15 00:00 [entrez]
AID - 0300-483X(95)03279-O [pii]
AID - 10.1016/0300-483x(95)03279-o [doi]
PST - ppublish
SO  - Toxicology. 1996 Apr 15;108(1-2):141-52. doi: 10.1016/0300-483x(95)03279-o.