PMID- 8645347
OWN - NLM
STAT- MEDLINE
DCOM- 19960715
LR  - 20190623
IS  - 0006-2952 (Print)
IS  - 0006-2952 (Linking)
VI  - 51
IP  - 9
DP  - 1996 May 3
TI  - Differential effects of phorbol ester on apoptosis in HL-60 promyelocytic 
      leukemia cells.
PG  - 1229-36
AB  - The role of the protein kinase C (PKC) activator phorbol 12-myristate 13-acetate 
      (PMA) in apoptosis of HL-60 cells was investigated. PMA inhibited DNA 
      fragmentation induced by thapsigargin (TG) and 4-bromo-calcium ionophore 
      (Br-A23187). The inhibitory effect of PMA was concentration-related and was 
      abolished by a specific PKC inhibitor, bisindolylmaleimide (GF109203X. In 
      addition TG-induced apoptosis was decreased in cells in which PKC activity was 
      down-regulated by long-term pretreatment with PMA. These results indicate that 
      PKC activation by PMA inhibits HL-60 cell apoptosis induced by TG and Br-A23187, 
      and that this inhibition is not influenced by the down-regulation of PKC. 
      However, PMA did not inhibit DNA fragmentation induced by 
      1-beta-D-arabinofuranosylcytosine (Ara-C) and cycloheximide. PMA suppressed TG- 
      or Br-A23187. Our results indicate that PKC participates in the regulation of 
      apoptosis only by some pathways. Down-regulation of PKC is not responsible for 
      the diverse effects of PKC activators on apoptosis. The effect of a PKC modulator 
      on apoptosis is dependent upon interaction with individual apoptotic stimulus.
FAU - Zhu, W H
AU  - Zhu WH
AD  - Department of Physiology, Faculty of Medicine, University of Hong Kong, Hong 
      Kong.
FAU - Loh, T T
AU  - Loh TT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem Pharmacol
JT  - Biochemical pharmacology
JID - 0101032
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Terpenes)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 67526-95-8 (Thapsigargin)
RN  - 76455-48-6 (4-bromo-A-23187)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Calcimycin/analogs & derivatives/pharmacology
MH  - Calcium/metabolism
MH  - Down-Regulation
MH  - Enzyme Inhibitors/pharmacology
MH  - HL-60 Cells
MH  - Homeostasis/drug effects
MH  - Humans
MH  - Protein Kinase C/antagonists & inhibitors/metabolism
MH  - Terpenes/antagonists & inhibitors/pharmacology
MH  - Tetradecanoylphorbol Acetate/*pharmacology
MH  - Thapsigargin
EDAT- 1996/05/03 00:00
MHDA- 1996/05/03 00:01
CRDT- 1996/05/03 00:00
PHST- 1996/05/03 00:00 [pubmed]
PHST- 1996/05/03 00:01 [medline]
PHST- 1996/05/03 00:00 [entrez]
AID - 0006295296000962 [pii]
AID - 10.1016/0006-2952(96)00096-2 [doi]
PST - ppublish
SO  - Biochem Pharmacol. 1996 May 3;51(9):1229-36. doi: 10.1016/0006-2952(96)00096-2.