PMID- 8646878
OWN - NLM
STAT- MEDLINE
DCOM- 19960722
LR  - 20180214
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 73
IP  - 1-2
DP  - 1996
TI  - Chromosomal evolution in duiker antelope (Cephalophinae: Bovidae): karyotype 
      comparisons, fluorescence in situ hybridization, and rampant X chromosome 
      variation.
PG  - 116-22
AB  - Fluorescence in situ hybridization (FISH) and conventional banding techniques 
      were used to identify patterns of similarity among the genomes of six species of 
      antelope, subfamily Cephalophinae. The G-banded euchromatic portions of the 
      autosomes were invariable in all species; however, significant modifications of 
      the X chromosomes were detected. Two of the taxa, Cephalophus maxwellii and C. 
      monticola, were characterized by acrocentric X's, while X chromosome morphology 
      varied from submetacentric to metacentric in the remaining species (C. dorsalis, 
      C. natalensis, Sylvicapra grimmia, and C. silvicultor). The short arm of the X 
      was heterochromatic in each species. Total genomic DNAs from these antelope were 
      used as hybridization probes against Cephalophus metaphase chromosomes and 
      resulted in robust fluorescence in the pericentromeric region of each autosome 
      and in the heterochromatic short arm of the X chromosome, indicating 
      complimentarity of DNA sequences in these regions. Conversely, chromosome 
      painting involving genomic DNAs derived from the subfamilies Alcelaphinae 
      (Pygargus dorcas) and Neotraginae (Oreotragus oreotragus) showed a marked absence 
      of hybridization at these sites. Additionally, X chromosome comparisons between 
      the Cephalophinae and Bovinae (represented by Bos taurus) revealed two 
      euchromatic pericentric inversions which had occurred since their common 
      ancestry. There is good G-band homoeology between the inverted cattle chromosome 
      region Xq12 --> q34 and most of the proximal portion of Xq in duikers, as well as 
      between the distal third of the duiker Xq and the cattle Xp. The latter 
      rearrangement was further confirmed by in situ hybridization using a probe 
      containing an insert spanning bands p12 to p14 of the cattle X chromosome.
FAU - Robinson, T J
AU  - Robinson TJ
AD  - Department of Zoology and Entomology, University of Pretoria, South Africa.
FAU - Wilson, V
AU  - Wilson V
FAU - Gallagher, D S Jr
AU  - Gallagher DS Jr
FAU - Taylor, J F
AU  - Taylor JF
FAU - Davis, S K
AU  - Davis SK
FAU - Harrison, W R
AU  - Harrison WR
FAU - Elder, F F
AU  - Elder FF
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
SB  - IM
MH  - Animals
MH  - Antelopes/classification/*genetics
MH  - Cattle/genetics
MH  - Cells, Cultured
MH  - Chromosome Banding/veterinary
MH  - *Evolution, Molecular
MH  - Female
MH  - Fibroblasts/ultrastructure
MH  - Genome
MH  - In Situ Hybridization, Fluorescence/veterinary
MH  - Karyotyping/veterinary
MH  - Male
MH  - Species Specificity
MH  - *X Chromosome/genetics/ultrastructure
EDAT- 1996/01/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1159/000134321 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1996;73(1-2):116-22. doi: 10.1159/000134321.