PMID- 8646895
OWN - NLM
STAT- MEDLINE
DCOM- 19960722
LR  - 20180214
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 73
IP  - 1-2
DP  - 1996
TI  - Assignment of the human tryptophanyl-tRNA synthetase gene (WARS) to chromosome 
      14q32.2 --> q32.32.
PG  - 99-103
AB  - Tryptophanyl-tRNA synthetase catalyzes the aminoacylation of tRNAtrp with 
      tryptophan, an essential function in the cell's protein synthesis machinery. It 
      has been shown that tryptophanyl-tRNA synthetase is induced by interferon, and 
      this has led to hypotheses about other possible functions of tryptophanyl-tRNA 
      synthetase. We have mapped a cDNA probe of the tryptophanyl-tRNA synthetase gene 
      (WARS) by a combination of somatic cell hybrid analysis, fluorescence in situ 
      hybridization (FISH), and linkage analysis. Both FISH and linkage analysis 
      independently supported a more distal position of WARS than had been previously 
      reported. FISH mapping indicated a most likely location at 14q32.31. Linkage 
      analysis was based on the 40 reference families from the CEPH collaboration and 
      resulted in a 13-point map, placing WARS, with odds of more than 1,000:1, within 
      an area of approximately 10 cM and, with odds of 198:1, in an approximately 6 cM 
      interval between pCMM101 and D14S27. The study provides additional integration of 
      the physical and genetic maps of the distal part of 14q, as well as genetic tools 
      enabling a more complete understanding of the function of tryptophanyl-tRNA 
      synthetase, especially with regard to the cryptic inducibility of interferon.
FAU - Borglum, A D
AU  - Borglum AD
AD  - Department of Human Genetics, Aarhus University, Denmark.
FAU - Flint, T
AU  - Flint T
FAU - Tommerup, N
AU  - Tommerup N
FAU - Fleckner, J
AU  - Fleckner J
FAU - Justesen, J
AU  - Justesen J
FAU - Kruse, T A
AU  - Kruse TA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (DNA, Complementary)
RN  - EC 6.1.1.2 (Tryptophan-tRNA Ligase)
SB  - IM
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 14/*genetics
MH  - DNA, Complementary/genetics
MH  - Female
MH  - Genes
MH  - Humans
MH  - Hybrid Cells
MH  - In Situ Hybridization, Fluorescence
MH  - Lod Score
MH  - Male
MH  - Pedigree
MH  - Tryptophan-tRNA Ligase/*genetics
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1159/000134317 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1996;73(1-2):99-103. doi: 10.1159/000134317.