PMID- 8647618
OWN - NLM
STAT- MEDLINE
DCOM- 19960722
LR  - 20160303
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 66
IP  - 5
DP  - 1996 May 29
TI  - Numerical aberration and point mutation of p53 gene in human gastric intestinal 
      metaplasia and well-differentiated adenocarcinoma: analysis by fluorescence in 
      situ hybridization (FISH) and PCR-SSCP.
PG  - 594-9
AB  - This study examined p53 gene alterations in human gastric mucosa, intestinal 
      metaplasia and well-differentiated (cohesive type) adenocarcinomas to clarify to 
      the role of the p53 gene in gastric tumorigenesis by means of fluorescence in 
      situ hybridization (FISH), polymerase-chain-reaction-single-strand-conformation 
      polymorphism (PCR-SSCP) and immunohistochemistry. Gene alterations were compared 
      with numerical changes of chromosome 17. Samples were obtained from 31 surgically 
      resected stomachs affected with gastric cancer. There was no nuclear p53 protein 
      in cells from normal gastric mucosa. Among 23 specimens of intestinal metaplasia, 
      cells with p53 protein were variably detected in 5 incomplete metaplasias 
      (colonic type). A histological study revealed a mildly dysplastic appearance. 
      PCR-SSCP identified p53-gene mutation in exons 5 and 8 in 2 of the 5 samples. 
      Numerical aberrations of chromosome 17 and the p53 gene were undetectable both in 
      normal mucosa and in intestinal metaplasia. Variable numbers of tumor cells 
      contained p53 protein in 13 (54%) of 24 carcinomas, and PCR-SSCP revealed 
      abnormal bands in 5 of them. Mutations were detected in exons 5, 7, 7, 7 and 8. 
      FISH analysis demonstrated that 6 carcinomas emitted 3 or 4 signals for 
      chromosome 17, and 7 gave one signal for the p53 gene in over 20% of the cells. 
      Ten (77%) of 13 carcinomas examined by FISH appeared to show a p53-gene deletion.
FAU - Gomyo, Y
AU  - Gomyo Y
AD  - First Department of Pathology, Faculty of Medicine, Tottori University, Japan.
FAU - Osaki, M
AU  - Osaki M
FAU - Kaibara, N
AU  - Kaibara N
FAU - Ito, H
AU  - Ito H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
MH  - Adenocarcinoma/*genetics/pathology
MH  - Aged
MH  - Base Sequence
MH  - Cell Differentiation/physiology
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 17
MH  - Female
MH  - Gastric Mucosa/*pathology/physiology
MH  - *Genes, p53
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Intestinal Mucosa/*pathology/physiology
MH  - Male
MH  - Metaplasia/genetics
MH  - Molecular Sequence Data
MH  - *Point Mutation
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Single-Stranded Conformational
MH  - Precancerous Conditions/*genetics
MH  - Stomach Neoplasms/*genetics/pathology
MH  - Tumor Suppressor Protein p53/analysis
EDAT- 1996/05/29 00:00
MHDA- 2000/06/20 09:00
CRDT- 1996/05/29 00:00
PHST- 1996/05/29 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1996/05/29 00:00 [entrez]
AID - 10.1002/(SICI)1097-0215(19960529)66:5<594::AID-IJC2>3.0.CO;2-O [pii]
AID - 10.1002/(SICI)1097-0215(19960529)66:5<594::AID-IJC2>3.0.CO;2-O [doi]
PST - ppublish
SO  - Int J Cancer. 1996 May 29;66(5):594-9. doi: 
      10.1002/(SICI)1097-0215(19960529)66:5<594::AID-IJC2>3.0.CO;2-O.