PMID- 8648756
OWN - NLM
STAT- MEDLINE
DCOM- 19960723
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 70
IP  - 6
DP  - 1996 Jun
TI  - Cloning, expression and characterization of the proteinase from human herpesvirus 
      6.
PG  - 4136-41
AB  - After the U53 gene encoding the proteinase from human herpesvirus 6 (HHV-6) was 
      sequenced, it was expressed in Escherichia coli, and the activity of the 
      purified, recombinant HHV-6 proteinase was characterized quantitatively by using 
      synthetic peptide substrates mimicking the release and maturation cleavage sites 
      in the polyprotein precursors of HHV-6, human cytomegalovirus (CMV), murine CMV, 
      and Epstein-Barr virus. Despite sharing 40% identity with other betaherpesvirus 
      proteinases such as human CMV proteinase, the one-chain HHV-6 enzyme was 
      distinguished from these two-chain proteinases by the absence of an internal 
      autocatalytic cleavage site.
FAU - Tigue, N J
AU  - Tigue NJ
AD  - Department of Molecular and Medical Biosciences, University of Wales College of 
      Cardiff, United Kingdom.
FAU - Matharu, P J
AU  - Matharu PJ
FAU - Roberts, N A
AU  - Roberts NA
FAU - Mills, J S
AU  - Mills JS
FAU - Kay, J
AU  - Kay J
FAU - Jupp, R
AU  - Jupp R
LA  - eng
SI  - GENBANK/X87419
PT  - Journal Article
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Recombinant Proteins)
RN  - EC 3.4.- (Endopeptidases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Endopeptidases/genetics/isolation & purification/*metabolism
MH  - Herpesvirus 6, Human/*enzymology
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Molecular Sequence Data
MH  - Rabbits
MH  - Recombinant Proteins/metabolism
PMC - PMC190303
EDAT- 1996/06/01 00:00
MHDA- 1996/06/01 00:01
PMCR- 1996/06/01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 1996/06/01 00:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
PHST- 1996/06/01 00:00 [pmc-release]
AID - 10.1128/JVI.70.6.4136-4141.1996 [doi]
PST - ppublish
SO  - J Virol. 1996 Jun;70(6):4136-41. doi: 10.1128/JVI.70.6.4136-4141.1996.