PMID- 8649434
OWN - NLM
STAT- MEDLINE
DCOM- 19960725
LR  - 20190825
IS  - 0161-5890 (Print)
IS  - 0161-5890 (Linking)
VI  - 33
IP  - 2
DP  - 1996 Feb
TI  - Regulation of the leukocyte integrin gene CD11c is mediated by AP1 and Ets 
      transcription factors.
PG  - 115-27
AB  - The leukocyte integrin gene, CD11c, encodes the chi subunit of the p150,95 
      (CD11c.CD18) receptor. Expression of the CD11c gene is predominately seen in 
      monocytes, but has also been detected in some B- and T-cell neoplasms and in some 
      large-cell lymphomas of uncertain origin. To elucidate the molecular mechanisms 
      that govern the expression of CD11c, we have cloned and characterized the 
      promoter region of this gene. The DNase I footprint and mobility shift analyses 
      revealed five sites within the -86 to +40 region that interact with nuclear 
      proteins. The -62 to -44 region contains two consensus sequences for AP1 
      (referred to as AP1-1 and AP1-2) and were shown to bind purified c-jun protein. 
      Co-transfection of c-fos and c-jun expression constructs with a CD11c 
      promoter-CAT fusion into HL60 cells led to a 6.7-fold increase in CD11c promoter 
      activity. We show that c-fos and c-jun mediate their effects through both AP1-1 
      and AP1-2 which function in an additive manner. Regions -42 to -34 and -13 to -5 
      contain consensus sequences for Ets factors (referred to as Ets C and Ets A, 
      respectively). Deletion of Ets resulted in a significant reduction in phorbol 
      ester-induced expression of CD11c, whereas deletion of Ets A led to only a modest 
      loss in CD11c expression. We show that Ets C cooperates with the AP1 sites to 
      regulate CD11c expression.
FAU - Noti, J D
AU  - Noti JD
AD  - Guthrie Research Institute, Sayre, PA 18840, USA.
FAU - Reinemann, C
AU  - Reinemann C
FAU - Petrus, M N
AU  - Petrus MN
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (DNA Primers)
RN  - 0 (Integrin alphaXbeta2)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-ets)
RN  - 0 (Transcription Factor AP-1)
RN  - 0 (Transcription Factors)
RN  - 9007-49-2 (DNA)
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
SB  - IM
MH  - Base Sequence
MH  - Binding Sites/genetics
MH  - Cell Line
MH  - Chloramphenicol O-Acetyltransferase/genetics
MH  - Cloning, Molecular
MH  - DNA/genetics/metabolism
MH  - DNA Footprinting
MH  - DNA Primers/genetics
MH  - *Gene Expression Regulation
MH  - Genes, fos
MH  - Genes, jun
MH  - Humans
MH  - Integrin alphaXbeta2/*genetics
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic
MH  - Protein Binding
MH  - Proto-Oncogene Proteins/*metabolism
MH  - Proto-Oncogene Proteins c-ets
MH  - Transcription Factor AP-1/*metabolism
MH  - Transcription Factors/*metabolism
EDAT- 1996/02/01 00:00
MHDA- 1996/02/01 00:01
CRDT- 1996/02/01 00:00
PHST- 1996/02/01 00:00 [pubmed]
PHST- 1996/02/01 00:01 [medline]
PHST- 1996/02/01 00:00 [entrez]
AID - 0161589095001409 [pii]
AID - 10.1016/0161-5890(95)00140-9 [doi]
PST - ppublish
SO  - Mol Immunol. 1996 Feb;33(2):115-27. doi: 10.1016/0161-5890(95)00140-9.