PMID- 8650724
OWN - NLM
STAT- MEDLINE
DCOM- 19960725
LR  - 20190727
IS  - 0039-2499 (Print)
IS  - 0039-2499 (Linking)
VI  - 27
IP  - 6
DP  - 1996 Jun
TI  - Confocal microscopic characterization of a lesion in a cerebral vessel of the 
      stroke-prone spontaneously hypertensive rat.
PG  - 1118-22; discussion 1122-3
AB  - BACKGROUND AND PURPOSE: Hypertension is a major risk factor for stroke and is 
      associated with alterations in vascular structure and function. The aim of this 
      study was to determine vascular function, wall morphology, and vascular smooth 
      muscle cell (VSMC) arrangement in basilar arteries from stroke-prone 
      spontaneously hypertensive rats (SHRSP) and normotensive control strain 
      Wistar-Kyoto rats (WKY). The effect of perindopril treatment on SHRSP structure 
      and function was also assessed. METHODS: VSMC orientation was determined with 
      laser-scanning confocal microscopy and computer-assisted image processing in 
      basilar arteries stained with 5(6)-carboxyfluorescein (wavelengths: excitation, 
      488; emission, 515) or propidium iodide (excitation, 529; emission, 550). 
      Measurements of wall morphology and functional responses to serotonin and KCl 
      were assessed with wire myography. RESULTS: In the WKY basilar arteries, VSMCs 
      were uniformly oriented perpendicular to the longitudinal axis of the vessel, 
      whereas in the SHRSP there were localized foci of VSMC geometric disorganization, 
      with a significant deviation from 90 degrees. The SHRSP basilar arteries also 
      showed structural remodeling and reduced contractile responses to serotonin and 
      KCl. Perindopril treatment normalized blood pressure, prevented wall morphology 
      alterations, and improved function but had no effect on VSMC disorganization. 
      CONCLUSIONS: This is the first demonstration of lesions of VSMC geometric 
      disorganization in a cerebral artery from a stroke-prone genetically hypertensive 
      rat strain. These structural abnormalities are independent of blood pressure. 
      Their functional sequel may play a role in the pathogenesis of stroke in this 
      model.
FAU - Arribas, S M
AU  - Arribas SM
AD  - Clinical Research Initiative in Heart Failure, University of Glasgow, UK. 
      s.arribas@biomed.gla.ac.uk
FAU - Gordon, J F
AU  - Gordon JF
FAU - Daly, C J
AU  - Daly CJ
FAU - Dominiczak, A F
AU  - Dominiczak AF
FAU - McGrath, J C
AU  - McGrath JC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Stroke
JT  - Stroke
JID - 0235266
RN  - 0 (Angiotensin-Converting Enzyme Inhibitors)
RN  - 0 (Antihypertensive Agents)
RN  - 0 (Coloring Agents)
RN  - 0 (Fluoresceins)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indoles)
RN  - 0 (Vasoconstrictor Agents)
RN  - 3301-79-9 (6-carboxyfluorescein)
RN  - 333DO1RDJY (Serotonin)
RN  - 36015-30-2 (Propidium)
RN  - 660YQ98I10 (Potassium Chloride)
RN  - Y5GMK36KGY (Perindopril)
SB  - IM
MH  - Angiotensin-Converting Enzyme Inhibitors/pharmacology
MH  - Animals
MH  - Antihypertensive Agents/pharmacology
MH  - Basilar Artery/drug effects/*pathology/physiopathology
MH  - Blood Pressure/drug effects
MH  - Cerebrovascular Disorders/*pathology/physiopathology
MH  - Coloring Agents
MH  - Female
MH  - Fluoresceins
MH  - Fluorescent Dyes
MH  - Image Processing, Computer-Assisted
MH  - Indoles/pharmacology
MH  - Male
MH  - Microscopy, Confocal
MH  - Muscle, Smooth, Vascular/drug effects/pathology/physiopathology
MH  - Perindopril
MH  - Potassium Chloride/pharmacology
MH  - Propidium
MH  - Rats
MH  - Rats, Inbred SHR
MH  - Rats, Inbred WKY
MH  - Serotonin/pharmacology
MH  - Vasoconstrictor Agents/pharmacology
EDAT- 1996/06/01 00:00
MHDA- 1996/06/01 00:01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 1996/06/01 00:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
AID - 10.1161/01.str.27.6.1118 [doi]
PST - ppublish
SO  - Stroke. 1996 Jun;27(6):1118-22; discussion 1122-3. doi: 10.1161/01.str.27.6.1118.